Genetic analysis of patients with hypertrophic cardiomyopathy using next generation sequencer

• Project/Area Number: 26860584
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Keio University
• Principal Investigator: Tanimoto Yoko 慶應義塾大学, 医学部, 助教 (50398616)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肥大型心筋症 / 遺伝子解析 / 次世代シークエンサー / 疾患特異的iPS細胞

Outline of Final Research Achievements

From the database consisted of HCM patients visiting Keio University Hospital, DNA samples were collected after obtaining written infromed consent. The DNAs were analyzed by the next generation sequencer. The identified variant was also confirmed by Sanger method. Furthermore, the iPS cells delived from these patients were established by the standard iPS cell preparation method. Each cell lines: healthy human derived iPS cell line and HCM derived iPS cells were shifted to mass culture system .

Research Products

• [Journal Article] Predictive factors of lead failure in patients implanted with cardiac devices (2015)
  • Author(s): Aizawa Y, Negishi M, Kashimura S, Nakajima K, Kunitomi A, Katsumata Y, Nishiyama T, Kimura T, Nishiyama N, Fukumoto K, Tanimoto Y, Kohsaka S, Takatsuki S, Fukuda K
  • Journal Title: Int J Cardiol Volume: 199 Pages: 277-81
  • Peer Reviewed
• [Journal Article] Visualization of the left atrial appendage by phased-array intracardiac echocardiography from the pulmonary artery in patients with atrial fibrillation. (2015)
  • Author(s): Nishiyama T, Katsumata Y, Inagawa K, Kimura T, Nishiyama N, Fukumoto K, Tanimoto Y, Aizawa Y, Tanimoto K, Fukuda K, Takatsuki S.
  • Journal Title: Europace Volume: 17 Issue: 4 Pages: 546-51
  • DOI: 10.1093/europace/euu383
  • Peer Reviewed
• [Journal Article] Risk factors for early replacement of cardiovascular implantable electronic devices (2015)
  • Author(s): Aizawa Y, Kunitomi A, Nakajima K, Kashimura S, Katsumata Y, Nishiyama T, Kimura T, Nishiyama N, Tanimoto Y, Kohsaka S, Takatsuki S, Fukuda K
  • Journal Title: Int J Cardiol Volume: 178 Pages: 99-101
  • DOI: 10.1016/j.ijcard.2014.10.157
  • Peer Reviewed
• [Journal Article] Operator-blinded contact force monitoring during pulmonary vein isolation using conventional and steerable sheaths. (2014)
  • Author(s): Kimura T, Takatsuki S, Oishi A, Negishi M, Kashimura S, Katsumata Y, Nishiyama T, Nishiyama N, Tanimoto Y, Aizawa Y, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 177 Issue: 3 Pages: 970-6
  • DOI: 10.1016/j.ijcard.2014.09.189
  • Peer Reviewed
• [Journal Article] Pericardial endoscopy-guided left atrial appendage ligation: a pilot study in a canine model. (2014)
  • Author(s): Kimura T, Takatsuki S, Miyoshi S, Katsumata Y, Nishiyama T, Nishiyama N, Tanimoto Y, Aizawa Y, Jinzaki M, Fukuda K.
  • Journal Title: Circ Cardiovasc Interv. Volume: 7 Issue: 6 Pages: 844-50
  • DOI: 10.1161/circinterventions.114.001610
  • Peer Reviewed
• [Journal Article] Thoracic impedance as a therapeutic marker of acute decompensated heart failure. (2014)
  • Author(s): Aizawa Y, Takatsuki S, Kashimura S, Katsumata Y, Nishiyama T, Kimura T, Nishiyama N, Tanimoto Y, Tanimoto K, Fukuda Y, Sato T, Ogawa S, Fukuda K.
  • Journal Title: Int J Cardiol Volume: 174 Issue: 3 Pages: 840-2
  • DOI: 10.1016/j.ijcard.2014.04.159
  • Peer Reviewed
• [Journal Article] Serum Inflammation Markers Predicting Successful Initial Catheter Ablation for Atrial Fibrillation. (2014)
  • Author(s): Kimura T, Takatsuki S, Inagawa K, Katsumata Y, Nishiyama T, Nishiyama N, Fukumoto K, Aizawa Y, Tanimoto Y, Tanimoto K, Fukuda K.
  • Journal Title: Heart Lung Circ. Volume: 14 Issue: 7 Pages: 636-43
  • DOI: 10.1016/j.hlc.2014.02.003
  • Peer Reviewed
• [Journal Article] Thrombus formation in the left atrial appendage during catheter ablation for atrial fibrillation under sufficient heparinization. (2014)
  • Author(s): Kimura T, Takatsuki S, Tanimoto K, Katsumata Y, Nishiyama T, Inagawa K, Nishiyama N, Tanimoto Y, Aizawa Y, Fukuda K.
  • Journal Title: Can J Cardiol. Volume: 30 Issue: 4 Pages: 465-465
  • DOI: 10.1016/j.cjca.2014.01.008
  • Peer Reviewed
• [Journal Article] Clinical characteristics of atrial fibrillation detected by implanted devices and its association with ICD therapy (2014)
  • Author(s): Aizawa Y
  • Journal Title: Int J Cardiol. Volume: 172 Issue: 3 Pages: 529-30
  • DOI: 10.1016/j.ijcard.2014.01.067
  • Peer Reviewed
• [Presentation] Clinical Characteristics, and Long-term Outcome of the Dilated Phase of Hypertrophic Cardiomyopathy (2016)
  • Author(s): Yoshiyasu Aizawa, Yoko Tanimoto , Mitsushige Murata , Mai Kimura , Yoshinori Katsumata , Takahiko Nishiyama , Takehiro Kimura , Nobuhiro Nishiyama , Kotaro Fukumoto , Shinsuke Yuasa , Takashi Kohno , Shun Kohsaka , Shinji Makino , Motoaki Sano , Seiji Takatsuki , Keiichi Fukuda
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 国際センター会議棟（宮城県仙台市）
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] High Incidence of Lead Failure in Patients with Brugada Syndrome and Idiopathic Ventricular Fibrillation (2016)
  • Author(s): Yoshiyasu Aizawa, Seiji Takatsuki , Kazuaki Nakajima , Shin Kashimura , Akira Kunitomi , Yoshinori Katsumata , Takahiko Nishiyama , Takehiro Kimura , Nobuhiro Nishiyama , Kotaro Fukumoto , Yoko Tanimoto , Shun Kohsaka , Keiichi Fukuda
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 国際センター展示棟（宮城県仙台市）
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] デバイス植込み患者における睡眠呼吸障害の合併状況と予測因子の検討 (2015)
  • Author(s): 相澤 義泰、河野 隆志、中嶋 一晶、樫村 晋、國富 晃、勝俣 良紀、西山 崇比古、木村 雄弘、西山 信大、福本 耕太郎、谷本 陽子、香坂 俊、高月 誠司、福田 恵一
  • Organizer: 第63回日本心臓病学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-09-18
  • Int'l Joint Research
• [Presentation] Comparison of circadian, weekly and seasonal Comparison of circadian, weekly and seasonal variations between electrical storms versus single VF events in patients with Brugada syndrome (2015)
  • Author(s): Yoshiyasu Aizawa, Seiji Takatsuki, Yoshinori Katsumata, Takahiko Nishiyama, Nobuhiro Nishiyama, Kotaro Fukumoto, Yoko Tanimoto, Yoshiaki Kaneko, Shinichi Niwano, Takashi Kurita, Takeshi Mitsuhashi, Takashi Noda, Shiro Kamakura, Akihiko Shimizu, Minoru Horie, Yoshifusa Aizawa, Keiichi Fukuda
  • Organizer: 第30回日本不整脈学会学術大会・第32回日本心電学会学術集会
  • Place of Presentation: グランドプリンスホテル京都（京都府京都市）
  • Year and Date: 2015-07-28
  • Int'l Joint Research
• [Presentation] Sex-dependency of Sick Sinus Syndrome Phenotype in Female Members of Familial Brugada Syndrome (2015)
  • Author(s): Yoshiyasu Aizawa, Seiji Takatsuki, Seiko Ohno, Satoshi Nagase, Shogo Ito, Kazuaki Nakajima, Shin Kashimura, Akira Kunitomi, Yoshinori Katsumata, Takahiko Nishiyama, Takehiro Kimura, Nobuhiro Nishiyama, Yoko Tanimoto, Satoshi Ogawa, Kenjiro Kosaki, Keiichi Fukuda
  • Organizer: Heart Rhythm Society
  • Place of Presentation: ボストン（米国）
  • Year and Date: 2015-05-13
  • Int'l Joint Research
• [Presentation] 当院で発生した植込みデバイスのリード不全発生症例の臨床学的特徴 (2015)
  • Author(s): 相澤義泰、根岸壮親、西山愛光、上岡天湖、中嶋一晶、樫村晋、國富晃、勝俣良紀、西山崇比古、木村雄弘、西山信大、谷本陽子、香坂俊、高月誠司、福田恵一
  • Organizer: 第７回植込みデバイス関連冬季大会
  • Place of Presentation: 東京都港区
  • Year and Date: 2015-02-21
• [Presentation] ラミンA/C変異を認めた家族性洞不全症候群の一例 (2015)
  • Author(s): 中嶋一晶、相澤義泰、伊藤章吾、樫村晋、勝俣良紀、西山崇比古、木村雄弘、西山信大、谷本陽子、湯浅慎介、小崎健次郎、福田恵一、高月誠司
  • Organizer: 第27回臨床不整脈研究会
  • Place of Presentation: 東京都港区
  • Year and Date: 2015-01-10
• [Presentation] 植え込みデバイスのジェネレータ交換頻度に寄与する臨床的指標の検討 (2014)
  • Author(s): 相澤義泰、高月誠司、樫村晋、勝俣良紀、西山崇比古、木村雄弘、西山信大、谷本陽子、谷本耕司郎、香坂俊、福田恵一
  • Organizer: 第62回日本心臓病学会学術集会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2014-09-26