| Project/Area Number |
26860586
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cardiovascular medicine
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
Endo Jin 慶應義塾大学, 医学部, 助教 (50398608)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 脂肪酸 / 心臓リモデリング / 骨髄由来細胞 / リピドミクス / 受容体探索 / 脂肪毒性 / ω-３脂肪酸 |
|---|
| Outline of Final Research Achievements |
We revealed that the fatty acid composition in each organ and tissue impacted their functional activities and the enrichment of omega-3 fatty acids in bone marrow-derived cells played a key role in the cardioprotective effects of omega-3 fatty acids under pressure overload. LC-MS/MS-based lipidomic analysis of lipid mediator identified 18-HEPE as a major EPA metabolite released by the cardiac macrophages in omega-3 enriched heart. 18-HEPE, which exhibited anti-fibrotic and anti-inflammatory properties in vitro, prevented the pressure overload-induced cardiac remodeling in vivo. These research findings were published in a scientific journal（Endo J et al. J Exp Med. 211(8), 1673-87, 2014）.
To identify an 18-HEPE specific receptor, we are now searching the cell lines and the stimulants optimized for the receptor screening. Using MS-imaging technique, we are examining the significance of lipid distribution in the pathological heart tissue.
|
