Development of Novel HDL Mimetic Fluorescent Peptide and Establishment of molecular Biomarker for Atherosclerosis

• Project/Area Number: 26860592
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Fukuoka University
• Principal Investigator: Kawachi Emi 福岡大学, 医学部, 助教 (50714416)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ペプチド / アポA-1 / 動脈硬化 / HDL / アポA-１

Outline of Final Research Achievements

We developed the peptide which can be used for PET imaging to visualize aortic plaque. In order to clarify the mechanism of action and the target molecule of FAMP, we successfully synthesized FITC-FAMP which possesses same functionality with FAMP. We supposed FAMP which adheres to the monocyte is transported to the atherosclerotic lesions, and integrated into the site of inflammation or plaques. However, neutrophil was positive with FITC-FAMP in the FACS system. It was suggested that FAMP adheres to neutrophils in blood and accumulates into the sites of inflammation or arteriosclerotic lesions.

Research Products

• [Journal Article] Newly developed apolipoprotein A-I mimetic peptide promotes macrophage reverse cholesterol transport in vivo. (2015)
  • Author(s): Shimizu T, Tanigawa H, Miura S, Kuwano T, Takata K, Suematsu Y, Imaizumi S, Yahiro E, Zhang B, Uehara Y, Saku K.
  • Journal Title: Int J Cardiol. Volume: 192 Pages: 82-88
  • DOI: 10.1016/j.ijcard.2016.01.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of 64Cu and 68Ga for Molecular Imaging of Atherosclerosis using the Apolipoprotein A-I Mimetic Peptide FAMP (2015)
  • Author(s): Yahiro, Eiji; Kawachi, Emi; Miura, Shin-Ichiro; Kuwano, Takashi; Imaizumi, Satoshi; Iwata, Atsushi; Hasegawa, Koki; Yano, Tsuneo; Watanabe, Yasuyoshi; Uehara, Yoshinari; Saku, Keijiro
  • Journal Title: Journal of Cardiovascular Diseases & Diagnosis Volume: 3 Issue: 03 Pages: 1-6
  • DOI: 10.4172/2329-9517.1000201
  • Peer Reviewed / Open Access
• [Journal Article] Improved survival rate after myocardial infarction using an inducible cholesterol efflux (iCE) peptide: FAMP (2014)
  • Author(s): Eiji Yahiro, Yoshinari Uehara, Emi Kawachi, Setsuko Ando, Shin-ichiro Miura, Keijiro Saku
  • Journal Title: IJC Heart & Vessels Volume: 4 Pages: 135-137
  • Peer Reviewed / Open Access
• [Journal Article] Impact of Cigarette Smoking Cessation on High-Density Lipoprotein Functionality －VN-SEESAW-HDL－ (2014)
  • Author(s): Kohei Takata, Satoshi Imaizumi, Emi Kawachi, Yasunori Suematsu, Tomohiko Shimizu, Satomi Abe, Yoshino Matsuo, Hitomi Tsukahara, Keita Noda, Eiji Yahiro, Bo Zhang, Yoshinari Uehara, Shin-ichiro Miura, Keijiro Saku
  • Journal Title: Circulation Journal Volume: 78 Pages: 2955-2962
  • NAID: 130004699203
  • Peer Reviewed
• [Presentation] Inhibition of lipopolysaccharide induced gene expression by HDL from healthy subjects and patients with acute myocardial infarction. (2016)
  • Author(s): Imaizumi S, Takata K, Yahiro E, Kawachi E, Iwata A, Uehara Y, Zhang Bo, Miura S, Saku K
  • Organizer: ESC congree
  • Place of Presentation: イタリア
  • Year and Date: 2016-08-26
• [Presentation] Effect of Statins on the Relationships of Adiponectin and Lipoproteins Analyzed by Capillary Isotachophoresis. (2015)
  • Author(s): Takata K, Imaizumi S, Zhang B, Kawachi E, Uehara Y, Miura S, Saku K
  • Organizer: The 79th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-24
• [Presentation] FAMP (Fukuoka University Apo A-1 Mimetic peptide) ameliorates impaired HDL function with activation of NO related pathway. (2015)
  • Author(s): Takata K, Imaizumi S, Kawachi E, Yahiro E, Zhang B, Uehara Y, Miura S, Saku K
  • Organizer: The 79th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-24
• [Presentation] A newly developed apoA-I mimetic peptide with a D-amino acid promotes HDL via ABCA1-mediated cholesterol efflux (2014)
  • Author(s): Emi Kawachi, Yoshinari Uehara, Eiji Yahiro, Satomi Abe, Setsuko Ando, Shin-Ichiro Miura, Keijiro Saku
  • Organizer: European Society of Cardiology
  • Place of Presentation: スペイン
  • Year and Date: 2014-08-28 – 2014-09-02
• [Presentation] Effect of smoking cessation on HDL function (2014)
  • Author(s): Emi Kawachi, Satoshi Imaizumi, Kohei Takata, Satomi Abe, Yoshino Matsuo, Eiji Yahiro, Yoshinari Uehara, Bo Zhang, Shin-Ichiro Miura, Keijiro Saku
  • Organizer: European Society of Cardiology
  • Place of Presentation: スペイン
  • Year and Date: 2014-08-28 – 2014-09-02