Role of Nitric Oxide Synthases in Hypoxic Pulmonary Hypertension in Mice
| Project/Area Number |
26860620
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | University of Occupational and Environmental Health, Japan |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 肺高血圧 / 一酸化窒素 / Nitric oxide / Pulmonary Hypertension / Bone marrow / Nitric oxide synthase / 低酸素性肺高血圧 / 一酸化窒素合成酵素 / NOS |
|---|
| Outline of Final Research Achievements |
The role of nitric oxide synthases (NOSs) in the development of pulmonary hypertension (PH) has been examined in pharmacological studies with non-selective NOSs inhibitors. However, due to the non-specificity of the NOSs inhibitors, the authentic role of NOSs in PH still remains to be clarified. We show here that hypoxia-induced PH was markedly deteriorated in triply
n/i/eNOSs-/- genotype and, to a lesser extent, in eNOS-/- genotype, but not in nNOS-/- or
iNOS-/- genotype, as compared with WT genotype. In addition, transplantation of triply NOSs-/- bone marrow results in the exacerbation of hypoxia-induced PH in mice, demonstrating the protective role of myelocytic NOSs in the pathogenesis of PH. Our findings provide novel insights into the cellular and molecular bases of PH.
|
Report
(4 results)
Research Products
(9 results)
-
-
-
[Presentation] Protective Role of Myelocytic Nitric Oxide Synthases in Hypoxic Pulmonary Hypertension in Mice2016
Author(s)
Masato Tsutsui, Takaaki Ogoshi, Takashi Kido, Sohsuke Yamada, Ke-Yong Wang, Yumiko Toyohira, Hiroaki Shimokawa, Nobuyuki Yanagihara, Nobuyuki Yanagihara, Kazuhiro Yatera, Hiroshi Mukae
Organizer
American Heart AssociationScientific Sessions 2016
Place of Presentation
New Orleans Ernest N. Morial Convention Center, New Orleans, USA
Year and Date
2016-11-12
Related Report
Int'l Joint Research
-
[Presentation] Role of Myelocytic Nitric Oxide Synthases in Hypoxic Pulmonary Hypertension in Mice2016
Author(s)
Takaaki Ogoshi, Masato Tsutsui, Takashi Kido, Sohsuke Yamada, Ke-Yong Wang, Yumiko Toyohira, Hiroaki Shimokawa, Nobuyuki Yanagihara, Nobuyuki Yanagihara, Kazuhiro Yatera, Hiroshi Mukae
Organizer
The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
Place of Presentation
Sendai International center, Sendai, Japan
Year and Date
2016-05-20
Related Report
Int'l Joint Research
-
-
-
-
-
