Analysis of kidney-specific transcriptional regulation of Epo gene and establishment of mouse model of renal anemia
Project/Area Number |
26860625
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Tohoku University |
Principal Investigator |
Hirano Ikuo 東北大学, 医学系研究科, 助教 (00708117)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | エリスロポエチン / 低酸素ストレス / 転写調節機構 / 腎性貧血 / 転写制御機構 / 慢性貧血モデル / 慢性腎臓病 / 遺伝子発現制御 / Kidney / Erythropoietin / transgenic mouse / hypoxia |
Outline of Final Research Achievements |
We performed transgenic reporter mouse analyses to analyze a mechanism of renal Epo gene regulation. We have found that a 13.8-kbp region (CURE region), spanning the region from 17.4 kb to 3.6 kb upstream of the Epo gene, is necessary for renal Epo gene regulation. we have also found that mice lacking the CURE region suffer from severe anemia caused by the EPO-deficiency. We named the anemic mice “AnRED (anemic model with renal EPO deficiency) mice”. CURE region harbors several phylogenetically conserved elements between humans and mice (among mammals). Our results obtained by transgenic reporter mouse analyses show that these elements redundantly and cooperatively work for the regulation of Epo gene in renal EPO-producing cells.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Erythropoietin Synthesis in Renal Myofibroblasts Is Restored by Activation of Hypoxia Signaling2016
Author(s)
Tomokazu Souma, Masahiro Nezu, Daisuke Nakano, Shun Yamazaki, Ikuo Hirano, Hiroki Sekine, Takashi Dan, Kotaro Takeda, Guo-Hua Fong, Akira Nishiyama, Sadayoshi Ito, Toshio Miyata, Masayuki Yamamoto, Norio Suzuki
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Journal Title
Journal of the american society of nephlorogy
Volume: 27
Issue: 2
Pages: 428-438
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes.2015
Author(s)
Tojo Y, Sekine H, Hirano I, Pan X, Souma T, Tsujita T, Kawaguchi S, Takeda N, Takeda K, Fong GH, Dan T, Ichinose M, Miyata T, Yamamoto M, Suzuki N.
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Journal Title
Mol Cell Biol.
Volume: 35
Issue: 15
Pages: 2658-2672
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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