| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
During development, the nephron is generated through a delicate balance between propagation and differentiation of nephron progenitors; however, these progenitors cease proliferation and terminally differentiate around the time of birth (a few days after birth in mice and a few weeks before birth in humans). No new nephrons are formed in the adult kidney, which may explain the irreversible nature of chronic kidney disease. In this study, we defined culture conditions for nephron progenitor propagation including FGF9, BMP7, and a WNT-activating reagent, with the addition of leukemia inhibitory factor (LIF). Under these tightly regulated culture conditions, mouse embryonic progenitors proliferated beyond the physiological limits observed in vivo, both for cell number and lifespan (1,800 fold within 19 days), and upon administration of the differentiation cue, they readily formed three-dimensional glomeruli and renal tubules.
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