Re-expression of Sall1 in podocytes protects against adriamycin-induced nephrosis
Project/Area Number |
26860648
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Juntendo University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ポドサイト / Sall1 / 糸球体腎炎 / アポトーシス / シナプトポディン / GRP78 / アドリアマイシン腎症 / ERストレス / Sall 1 / 糸球体硬化 |
Outline of Final Research Achievements |
Sall1 has a crucial role in kidney development. To explore this role in differentiated podocytes, we generated podocyte-specific Sall1-deficient mice (Sall1 KO p/p) and siRNA Sall1 knock down (Sall1 KD) podocytes. Sall1 KO p/p mice showed no proteinuria, however after adriamycin treatment (ADRTx) they showed much of proteinuria and many sclerotic glomeruli. Sall1 KD podocytes showed a loss of synaptopodin following ADRTx. These results indicated that Sall1 has a protective role in podocytes; to explore this mechanism we focused on GRP78. GRP78 expression was higher in ADRTx-Sall1 KO p/p mice than in control mice. Sall1 appeared to influence the expression of GRP78 in injured podocytes. These results suggest that Sall1 is associated with actin re-organization, endoplasmic reticulum stress, and apoptosis in injured podocytes. These protective aspects of Sall1 re-expression in injured podocytes may have the potential to reduce apoptosis and possibly glomerulosclerosis.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Re-expression of Sall1 in podocytes protects against adriamycin-induced nephrosis2017
Author(s)
Yoshiko Hosoe-Nagai, Teruo Hidaka, Ayano Sonoda, Yu Sasaki, Kanae Yamamoto-Nonaka, Takuto Seki, Rin Asao, Eriko Tanaka, Juan Alejandro Oliva Trejo, Fumiko Kodama, Miyuki Takagi, Nobuhiro Tada, Takashi Ueno, Ryuichi Nishinakamura, Yasuhiko Tomino, and Katsuhiko Asanuma
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Journal Title
Laboratory Investigation
Volume: 未定
Pages: 未定
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Notch2 activation ameliorates nephrosis.2014
Author(s)
Tanaka E, Asanuma K, Kim E, Sasaki Y, Oliva Trejo JA, Seki T, Nonaka K, Asao R, Nagai-Hosoe Y, Akiba-Takagi M, Hidaka T, Takagi M, Koyanagi A, Mizutani S, Yagita H, Tomino Y.
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Journal Title
Nature Communications
Volume: 5
Pages: 3296
DOI
Related Report
Peer Reviewed / Open Access
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