Research Project
Grant-in-Aid for Young Scientists (B)
Alpha-synuclein plays an important role in the development of Parkinson’s disease. In this study, we developed a novel method to quantify and visualize aggregated alpha-synuclein molecules using Raman microspectroscopy. Using this, we obtained Raman spectroscopic image of alpha-synuclein aggregates formed in vitro and in the culture cells. Vibrational spectra obtained using Raman spectroscopy provides vibrational information characteristic of chemical groups in a molecule. Measuring vibrational spectra enables us to locate and quantitate alpha-synuclein within tissues in a molecular specific manner. The alpha-synuclein aggregates formed in both conditions showed identical Raman spectroscopic patterns and they are different from those of monomeric alpha-synuclein. As Raman spectroscopy is label-free technique, the Raman shifts which is specific to aggregated alpha-synuclein molecules will work as a molecular specific marker within a physiological condition in vivo.
All 2017 2016 2015 2014
All Journal Article (1 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 1 results, Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 3 results, Invited: 1 results) Patent(Industrial Property Rights) (2 results)
Neurol Genet.
Volume: 2 Issue: 1 Pages: 1-9
10.1212/nxg.0000000000000048
