Development of label-free vibrational spectroscopy to quantify and visualize alpha-synuclein molecules in vivo
| Project/Area Number |
26860660
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Nagashima Yu 東京大学, 大学院理学系研究科(理学部), 特任研究員 (20635586)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | シヌクレイン / パーキンソン病 / ラマン分光法 / α-シヌクレイン / ラマン分光 / 振動スペクトル |
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| Outline of Final Research Achievements |
Alpha-synuclein plays an important role in the development of Parkinson’s disease. In this study, we developed a novel method to quantify and visualize aggregated alpha-synuclein molecules using Raman microspectroscopy. Using this, we obtained Raman spectroscopic image of alpha-synuclein aggregates formed in vitro and in the culture cells. Vibrational spectra obtained using Raman spectroscopy provides vibrational information characteristic of chemical groups in a molecule. Measuring vibrational spectra enables us to locate and quantitate alpha-synuclein within tissues in a molecular specific manner. The alpha-synuclein aggregates formed in both conditions showed identical Raman spectroscopic patterns and they are different from those of monomeric alpha-synuclein. As Raman spectroscopy is label-free technique, the Raman shifts which is specific to aggregated alpha-synuclein molecules will work as a molecular specific marker within a physiological condition in vivo.
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Atypical parkinsonism caused by Pro105Leu mutation of prion protein: A broad clinical spectrum.2016
Author(s)
Mano KK, Matsukawa T, Mitsui J, Ishiura H, Tokushige S, Takahashi Y, Sato NS, Nakamoto FK, Ichikawa Y, Nagashima Y, Terao Y, Shimizu J, Hamada M, Uesaka Y, Oyama G, Ogawa G, Yoshimura J, Doi K, Morishita S, Tsuji S, Goto J.
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Journal Title
Neurol Genet.
Volume: 2
Issue: 1
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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