Establishment of anti prion therapy by autophagy inducers
| Project/Area Number |
26860671
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAGAKI Takehiro 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (80722917)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | FK506 / プリオン病 / オートファジー / 神経変性疾患 |
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| Outline of Final Research Achievements |
We focused on autophagy that is degradation pathway of abnormal prion protein (PrPSc) as a therapeutic target of prion diseases. Previously, we reported that FK506 can be a therapeutic agent by activating autophagy. The most known regulator of autophagy is mammalian Target Of Rapamycin(mTOR). Torin1 and Torin2 which inhibit mTOR are well known autophagy inducer. We compared the anti prion effect of autophagy inducers(FK506, Torin1 and Torin2).Whereas Torin 1 and Torin2 they are well known autophagy inducers decreased PrPSc by about 50% in autophagy sensitive prion strain (Fukuoka-1 strain) infected cells, they did not reduce PrPSc in autophagy resistant strain (22L strain) infected cells. On the other hand, FK506 reduced PrPSc by about 80% in the cells with both Fukuoka-1 and 22L strains. These results suggested that FK506 has anti-prion effect stronger than already known autophagy inducers.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Characterisation of radioiodinated flavonoid derivatives for SPECT imaging of cerebral prion deposits.2015
Author(s)
Fuchigami T, Yamashita Y, Kawasaki M, Ogawa A, Haratake M, Atarashi R, Sano K, Nakagaki T, Ubagai K, Ono M, Yoshida S, Nishida N, Nakayama M.
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Journal Title
Sci Rep.
Volume: 5
Issue: 1
Pages: 18440-18440
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Rapid and Quantitative Assay of Amyloid-Seeding Activity in Human Brains Affected with Prion Diseases.2015
Author(s)
Takatsuki H, Satoh K, Sano K, Fuse T, Nakagaki T, Mori T, Ishibashi D, Mihara B, Takao M, Iwasaki Y, Yoshida M, Atarashi R, Nishida N.
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Journal Title
PLoS One
Volume: 10
Issue: 6
Pages: 0126930-0126930
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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