Lipidomic analysis of metabolic syndrome : Development of a sensitive liquid chromatography-mass spectrometry method for triacylglycerol

• Project/Area Number: 26860686
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Akita University
• Principal Investigator: Noriko Ueno 秋田大学, 学内共同利用施設等, 研究員 (90546631)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: トリアシルグリセロール / 質量分析 / リピドミクス / 中性脂質

Outline of Final Research Achievements

High levels of triacylglycerols (TAG) in the blood are associated with risk of the metabolic syndrome. It is hard to do the quantitative analysis of TAG molecular species using currently available methods, although it can apply to the quantitative analysis of total TAG and/or its subclass. In this study, I developed a novel analysis method, which is a liquid chromatography-mass spectrometry (LC-MS) for the high separative, high sensitive and qualitative TAG analysis. Furthermore, this method is able to also measure many lipid metabolites such as phospholipid and cholesteryl ester, diacylglycerol. In future, I aim at the elucidation of a biological phenomenon by global and/or specific comprehension of biological lipids using established method.

Research Products

• [Journal Article] INPP4B is a PtdIns(3,4,5)P3 phosphatase that can act as a tumor suppressor. (2015)
  • Author(s): Kofuji S, Kimura H, Nakanishi H, Nanjo H, Takasuga S, Liu H, Eguchi S, Nakamura R, Itoh R, Ueno N, Asanuma K, Huang M, Koizumi A, Habuchi T, Yamazaki M, Suzuki A, Sasaki J, Sasaki T
  • Journal Title: Cancer Discovery Volume: in press Issue: 7 Pages: 730-9
  • DOI: 10.1158/2159-8290.cd-14-1329
  • Peer Reviewed / Open Access