| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Megakaryocytes (MKs) differentiate from hematopoietic stem cells under the control of MK-specific cytokine, thrombopoietin (TPO). Subsequent maturation of MKs is morphologically characterized by polyploidization and expansion of the cytoplasmic body. Final stage of differentiation is characterized by platelet release from the ends of long thin cytoplasmic processes called proplatelet. However, the mechanism of proplatelet formation and platelet function have not been completely understood.
In this study, proplatelet formation, MK ploidy and platelet activation including platelet aggregation of dnam-1, calpastatin KO platelets were changed comparing with those of wild-type. These results suggest that DNAM-1, calpain-calpastatin are involved in megakaryopoiesis and platelet function. Furthermore, we demonstrated that sodium bicarbonate facilitates hemostasis.
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