| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We successfully generated multiple iPS cell lines from MDS clones (MDS-iPSC lines) of several MDS patients with abnormal karyotypes. We assessed hematopoietic differentiation potential of MDS-iPSC lines and isogenic normal T-iPSC lines. Hematopoietic colony formation in methylcellulose culture and further differentiation in erythroid and neutrophil culture were severely impaired in all tested MDS-iPSC lines.
Next, we performed microarray analysis in hematopoietic progenitor cells re-induced from MDS-iPSC lines and isogenic normal iPSC lines, identifying MDS-specific expression changes.
Finally, we performed whole-exome analysis to find that some of the somatic mutations detected in bulk cells of this MDS patient are shared in MDS-iPSC lines but not in isogenic normal iPSC lines.
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