| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Chronic myeloid leukemia is a myeloproliferative neoplasm related to the presence of the BCR-ABL fusion gene. It is originated from an abnormal hematopoietic stem cell. It is known that both leukemic and normal hematopoietic stem cells interact with bone marrow microenvironment. Although there have been many previous studies about bone marrow microenvironment, most of them were analyzed by conventional methods such as histological analysis and flow cytometry. These methods could not observe the dynamic cell movement in living bone marrow.In this study, I established the imaging system to observe the leukemia cell dynamics in the living bone marrow by using multiphoton microcopy. And I succeeded in visualization of leukeia cells moving. This technique would be useful not only for analysing the pathophysiology of leukemia but also for evaluating novel anti-cancer drugs.
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