Analysis for molecular role of Geminin in hematopoietic and leukemia stem cell
| Project/Area Number |
26860734
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Hiroshima University |
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Principal Investigator |
Ohno Yoshinori 広島大学, 原爆放射線医科学研究所, 助教 (10548986)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Geminin / 造血幹細胞 / 白血病幹細胞 |
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| Outline of Final Research Achievements |
Geminin negatively regulates DNA replication and chromatin remodeling through the direct interaction with Cdt1 and Brahma/Brg1, respectively. We previously demonstrated that Polycomb-group complex 1 and Hoxb4/Hoxa9 regulate the activity of hematopoietic stem cells (HSCs) through the ubiquitin proteasome system-mediated direct regulation of Geminin protein, and that Geminin suppresses the E2F-mediated transcriptional activity through the regulation of chromatin remodeling. Geminin is known as a regulator of DNA replication as well as chromatin remodeling. In this study, shRNA-mediated knockdown of Geminin and visualization of Geminin in Geminin-EYFP knockin mice further supported our hypothesis that Geminin acts as a key regulator for determining cell fate of HSCs, i.e., cellular quiescence, self-renewal and differentiation.
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Report
(3 results)
Research Products
(5 results)
