| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The hematopoietic stem cells (HSCs) possess both self-renewal and multi-lineage differentiation abilities and maintain lifelong hematopoiesis. It is generally considered that all of human HSC exist in the CD34-positive fraction. However, it is not clarified that the functional significance of the expression of CD34 antigen on the human hematopoietic stem cells. In order to elucidate this problem, I tried to the knock-out (KO) of CD34 antigen from human HSCs using CRISPR/Cas9 system. In the results, I succeeded to KO CD34 antigen from human HSC, though the frequency of KO was slightly low. Therefore, it is necessary to improve the efficiency of CD34 antigen KO system. The modification of CD34 KO system is now ongoing.
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