| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Microbiota may influence initiation and development of arthritis. We investigated the influence of long term anti-biotics (ABx) use on microbiota and the course of arthritis developed in IL-1ra -/- mice. ABx was given in the drinking water after weaning. ABx treatment reduced total bacteria to 8 percent and arthritis showed temporary improvement, accompanying decrease of intra-articular neutrophil and macrophages. The reduction of inflammatory cells were explained by decreased intra-articular cytokines and chemokines such as CXCL1, MIP-1β, and RANTES, but not IL-17A. The mice treated with ABx after development of arthritis did not show improvement of arthritis. These results indicate importance of timing and interval of ABx treatment when we apply ABx to modulation of microbiota for treatment of arthritis.
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