A basic study about the immune regulation by thymus-derived regulatory T cells induced in vivo

• Project/Area Number: 26860748
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Kanazawa University
• Principal Investigator: Hamano Ryoko 金沢大学, 医学系, 協力研究員 (10623655)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 制御性T細胞 / 胸腺 / 炎症 / 抗原特異性

Outline of Final Research Achievements

It is reported that a combined i.v. administration of antigen and IL-2-anti-IL-2 Ab immune complexes (IL-2 ICs) efficiently expands antigen-specific Treg cells in the thymus and induces their migration into peripheral blood. In this study, we explored that the expanded antigen-specific Treg cells rapidly move into the antigen injected site in CCR2 dependent manner. Moreover, prior treatment with antigen and IL-2 ICs enhanced antigen-specific Treg-cell migration and inhibited delayed type hypersensitivity (DTH) reactions. Thus, the treatment with Ag and IL-2 ICs can efficiently expand Ag-specific Treg cells with the capacity to migrate and reduce localized immune responses.

Research Products

• [Journal Article] A case developing minimal change disease during the course of IgG4-related disease. (2015)
  • Author(s): Yamada K, Zoshima T, Ito K, Mizushima I, Hara S, Horita S, Nuka H, Hamano R, Fujii H, Yamagishi M, Kawano M.
  • Journal Title: Modern Rheumatology Volume: Mar 24 Pages: 1-4
  • Peer Reviewed
• [Journal Article] A case developing minimal change disease during the course of IgG4-related disease (2015)
  • Author(s): Yamada K, Zoshima T, Ito K, Mizushima I, Hara S, Horita S, Nuka H, Hamano R, Fujii H, Yamagishi M, Kawano M.
  • Journal Title: Modern Rheumatology Volume: なし Pages: 1-4
  • Peer Reviewed
• [Journal Article] Ag and IL-2 immune complexes efficiently expand Ag-specific Treg cells that migrate in response to chemokines and reduce localized immune responses. (2014)
  • Author(s): Hamano R, Baba T, Sasaki S, Tomaru U, Ishizu A, Kawano M, Yamagishi M, Mukaida N
  • Journal Title: Eur J Immunol Volume: 44 Issue: 4 Pages: 1005-1015
  • DOI: 10.1002/eji.201343434
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of coronary artery screening tests and intervention in hemodialysis patients. (2014)
  • Author(s): Daimon S, Mizushima I, Hamano R, Terai H.
  • Journal Title: Therapeutic apheresis and dialysis Volume: 18 Issue: 5 Pages: 443-449
  • DOI: 10.1111/1744-9987.12167
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of MT-2 cells as a human regulatory T cell-like cell line. (2014)
  • Author(s): Hamano R, Wu X, Wang Y, Oppenheim JJ, Chen X.
  • Journal Title: Cellular and molecular immunology Volume: なし Issue: 6 Pages: 1-3
  • DOI: 10.1038/cmi.2014.123
  • Peer Reviewed / Open Access