Development of analytical tools for elucidating functions of epidermal gd T cells

• Project/Area Number: 26860761
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Juntendo University
• Principal Investigator: SHIMURA Eri 順天堂大学, 医学部, 准教授 (30586342)
• Research Collaborator: NAKAE Susumu 東京大学, 医科学研究所, 准教授 (60450409)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 表皮gdT細胞 / 皮膚免疫 / gdT細胞 / アレルギー / 接触性皮膚炎 / 皮膚 / 皮膚免疫応答 / γδT細胞

Outline of Final Research Achievements

DETC (dendritic epidermal T cell), a γδ T cell present in the epidermis, has been shown to have an important role in skin diseases such as wound healing, skin cancer and contact dermatitis. However, the role of DETC in the skin immune response remains unclear. In the background it seemed that there was still no useful tool specialized for DETC analysis.
In this research, for the purpose of constructing analysis method for elucidating the function of DETC, we screened 4 genes that code for molecules expressed on DETC.

Research Products

• [Journal Article] Loss of Dok-1 and Dok-2 in mice causes severe experimental colitis accompanied by reduced expression of IL-17A and IL-22. (2016)
  • Author(s): Waseda M, Arimura S, Shimura E, Nakae S, and Yamanashi Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 478 Issue: 1 Pages: 135-142
  • DOI: 10.1016/j.bbrc.2016.07.079
  • Peer Reviewed
• [Journal Article] IL-25, IL-33 and TSLP receptor are not critical for development of experimental murine malaria (2016)
  • Author(s): Akiko Shibuia, Ayako Takamori, Mohammed E.M. Tolbaa, Aya Nambu, Eri Shimura, Sachiko Yamaguchi, Chizu Sanjoba, Hajime Suto, Katsuko Sudo, Ko Okumura, Sumio Sugano, Hideaki Morita, Hirohisa Saito, Kenji Matsumoto, Susumu Nakae
  • Journal Title: Biochemistry and Biophysics Reports Volume: 5 Pages: 191-195
  • DOI: 10.1016/j.bbrep.2015.12.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Potential role of myeloid cell/eosinophil-derived IL-17 in LPS-induced endotoxin shock. (2014)
  • Author(s): Shimura E, Shibui A, Narushima S, Nambu A, Yamaguchi S, Akitsu A, Leonard WJ, Iwakura Y, Matsumoto K, Suto H, Okumura K, Sudo K, Nakae S
  • Journal Title: Biochem Biophys Res Commun Volume: 453 Issue: 1 Pages: 1-6
  • DOI: 10.1016/j.bbrc.2014.09.004
  • Peer Reviewed / Open Access