Study on the risk evaluation of viral infection based on single nucleotide polymorphism (SNP) of mitochondrial antiviral signaling protein (MAVS)

• Project/Area Number: 26860766
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Hirosaki University
• Principal Investigator: Xing Fei 弘前大学, 医学(系)研究科(研究院), 研究員 (70711985)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: MAVS / SNP

Outline of Final Research Achievements

We tested effects of twelve of non-synonymous MAVS SNPs in the coding region with amino acid substitution from NCBI database. We found five of these SNPs exhibited functional alteration that four of them resulted in the inhibitory immune response and one leaded to the contrary effect. One thousand and thirty-two human genomic samples obtained from a mass examination were genotyped at these five SNPs, however, no one variation, neither homozygous nor heterozygous, was detected. We considered that these five SNPs do not reside in Japanese population and such variations of MAVS may result in serious immune diseases, especially infections.

Research Products

• [Journal Article] Alteration of Antiviral Signalling by Single Nucleotide Polymorphisms (SNPs) of Mitochondrial Antiviral Signalling Protein (MAVS). (2016)
  • Author(s): Xing F, Matsumiya T, Hayakari R, Yoshida H, Kawaguchi S, Takahashi I, Nakaji S, Imaizumi T.
  • Journal Title: PLoS One Volume: 11 Issue: 3 Pages: e0151173-e0151173
  • DOI: 10.1371/journal.pone.0151173
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: Roles of TRAF3 and TRAF5. (2015)
  • Author(s): Chiba Y, Matsumiya T, Satoh T, Hayakari R, Furudate K, Xing F, Yoshida H, Tanji K, Mizukami H, Imaizumi T, Ito E.
  • Journal Title: Biochem Biophys Res Commun Volume: 467 Issue: 2 Pages: 191-196
  • DOI: 10.1016/j.bbrc.2015.10.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Desferrioxamine, an iron chelator, inhibits CXCL10 expression induced by polyinosinic-polycytidylic acidin U373MG human astrocytoma cells. (2015)
  • Author(s): Imaizumi T, Sakashita N, Mushiga Y, Yoshida H, Hayakari R, Xing F, Wang L, Matsumiya T, Tanji K, Chiba Y, Furudate K, Kawaguchi S, Murakami M, Tanaka H.
  • Journal Title: Neurosci Res. Volume: in press Pages: 10-16
  • DOI: 10.1016/j.neures.2015.01.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ISG54 and ISG56 are induced by TLR3 signaling in U373MG human astrocytoma cells: Possible involvement in CXCL10 expression. (2014)
  • Author(s): Imaizumi T, Numata A, Yano C, Yoshida H, Meng P, Hayakari R, Xing F, Wang L,Matsumiya T, Tanji K, Tatsuta T, Murakami M, Tanaka H.
  • Journal Title: Neurosci Res Volume: 84 Pages: 34-42
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tumor necrosis factor-α synergistically enhances polyinosinic-polycytidylic acid-induced toll-like receptor 3 signaling in cultured normal human mesangial cells: possible involvement in the pathogenesis of lupus nephritis (2014)
  • Author(s): Imaizumi T, Aizawa T, Hayakari R, Xing F, Meng P, Tsuruga K, Matsumiya T, Yoshida H, Wang L, Tatsuta T, Tanaka H
  • Journal Title: Clin Exp Nephrol Volume: (online ahead of print) Issue: 1 Pages: 75-81
  • DOI: 10.1007/s10157-014-0956-3
  • Peer Reviewed / Open Access
• [Journal Article] Carnosic acid attenuates apoptosis induced by amyloid-β 1-42 or 1-43 in SH-SY5Y human neuroblastoma cells. (2014)
  • Author(s): Meng P, Yoshida H, Tanji K, Matsumiya T, Xing F, Hayakari R, Wang L, Tsuruga K, Tanaka H, Mimura J, Kosaka K, Itoh K, Takahashi I, Kawaguchi S, Imaizumi T.
  • Journal Title: Neurosci Res Volume: - Pages: 1-9
  • DOI: 10.1016/j.neures.2014.12.003
  • Peer Reviewed / Open Access
• [Presentation] Single Nucleotide Polymorphism (SNP) of Mitochondrial Antiviral Signaling protein (MAVS) (2015)
  • Author(s): Xing F, Matsumiya T, Hayakari R, Yoshida H, Takahashi I, Nakaji S, Imaizumi T
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Double-stranded RNA induces the expression of type I interferon-stimulated genes 54 and 56 in human astrocyte cell line (2014)
  • Author(s): Fei Xing, Tomoh Matsumiya, Ryo Hayakari, Hidemi Yoshida, Tadaatsu Imaizumi
  • Organizer: 12th International Congress of Neuroimmunology
  • Place of Presentation: MAINZ，Germany
  • Year and Date: 2014-11-08 – 2014-11-15
• [Presentation] Involvement of IKKα in type I interferon-independent phosphorylation of STAT1 in response to double-stranded RNA (2014)
  • Author(s): Fei Xing, Tomoh Matsumiya, Ryo Hayakari, Hidemi Yoshida, Tadaatsu Imaizumi
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館，京都市
  • Year and Date: 2014-10-15 – 2014-10-19
• [Presentation] I 型インターフェロン非依存的な STAT1 リン酸化における IKKα の役割の検討 (2014)
  • Author(s): 邢飛，松宮朋穂，早狩亮，吉田秀見，今泉忠淳
  • Organizer: 第 151 回弘前医学会例会
  • Place of Presentation: 八戸グランドホテル，八戸市
  • Year and Date: 2014-06-08