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Development study of next generation therapeutic vaccine for clinical application

Research Project

Project/Area Number 26860771
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Infectious disease medicine
Research InstitutionSaitama Medical University

Principal Investigator

TAKAGI AKIRA  埼玉医科大学, 医学部, 助手 (20536891)

Research Collaborator AKATSUKA Toshitaka  埼玉医科大学, 医学部, 教授 (30159321)
UCHIDA Tetsuya  埼玉医科大学, 医学部, 特任教授 (50176690)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
KeywordsCTL / ワクチン / C型肝炎ウイルス / 細胞傷害性試験 / FACS
Outline of Final Research Achievements

We developed a cytotoxic T cell induced vaccine using liposomes. As a result, we have established one vaccine candidate and a new cytotoxic T cell assay method as a vaccine evaluation method.
Immunization of mice with a peptide derived from hepatitis C virus as a candidate for vaccine bound to the surface of liposome induces extremely high cytotoxic T cell activity, marked production of CD 107a and IFN-gamma, which are the markers, In the challenge experiment we were able to completely eliminate the virus. A new measurement method is a method that does not use conventional radioactive isotope, it is a very sensitive method, there is no fear of exposure, it is a method that can measure multiparameter and it can be expected for clinical application.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Characterization of the flow cytometric assay for ex vivo monitoring of cytotoxicity mediated by antigen-specific cytotoxic T lymphocytes.2017

    • Author(s)
      Takagi, A., Y. Horiuchi, and M. Matsui.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 492 Issue: 1 Pages: 27-32

    • DOI

      10.1016/j.bbrc.2017.08.045

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of universal HCV vaccine by coupling HCV NS3 protein to the surface of loposomes.2015

    • Author(s)
      高木 徹、小林 信春、松井 政則、堀内 大、持田 智、小林 清子、池淵 研二、赤塚 俊隆
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of universal HCV vaccine by coupling HCV NS3 protein to the surface of loposomes.2015

    • Author(s)
      高木 徹、小林 信春、松井 政則、堀内 大、赤塚 俊隆
    • Organizer
      第44回日本免疫学会総会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] リポソーム表面結合HCV-NS3タンパクによるユニバーサルワクチンの開発2015

    • Author(s)
      高木 徹、小林 信春、松井 政則、堀内 大、赤塚 俊隆
    • Organizer
      第19回日本ワクチン学会学術集会
    • Place of Presentation
      名鉄犬山ホテル(愛知県)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of universal HCV vaccine by coupling HCV NS3 protein to the surface of liposomes2015

    • Author(s)
      高木 徹、小林 信春、松井 政則、堀内 大、Ajit Kumar、赤塚 俊隆
    • Organizer
      22nd International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Strasbourg convention center(フランス)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of universal HCV vaccine by coupling HCV NS3 proteins to the surface of liposomes.2015

    • Author(s)
      高木 徹、小林 信春、堀内 大、持田 智、小林 清子、池淵 研二、Ajit Kumar、 種市 麻衣子、内田 哲也、赤塚 俊隆
    • Organizer
      15th International Symposium on Viral Hepatitis and Liver Disease
    • Place of Presentation
      Estrel Berlin-Hotel & Convention Center (ドイツ)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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