| Project/Area Number |
26860771
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
TAKAGI AKIRA 埼玉医科大学, 医学部, 助手 (20536891)
|
|---|
| Research Collaborator |
AKATSUKA Toshitaka 埼玉医科大学, 医学部, 教授 (30159321)
UCHIDA Tetsuya 埼玉医科大学, 医学部, 特任教授 (50176690)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
|---|
| Keywords | CTL / ワクチン / C型肝炎ウイルス / 細胞傷害性試験 / FACS |
|---|
| Outline of Final Research Achievements |
We developed a cytotoxic T cell induced vaccine using liposomes. As a result, we have established one vaccine candidate and a new cytotoxic T cell assay method as a vaccine evaluation method.
Immunization of mice with a peptide derived from hepatitis C virus as a candidate for vaccine bound to the surface of liposome induces extremely high cytotoxic T cell activity, marked production of CD 107a and IFN-gamma, which are the markers, In the challenge experiment we were able to completely eliminate the virus. A new measurement method is a method that does not use conventional radioactive isotope, it is a very sensitive method, there is no fear of exposure, it is a method that can measure multiparameter and it can be expected for clinical application.
|
