Analysis of immune escape mechanism of novel HIV-2 variants isolated from AIDS patients

• Project/Area Number: 26860777
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Okayama University (2015); National Hospital Organization Nagoya Medical Center (2014)
• Principal Investigator: Nemoto Michiko 岡山大学, その他の研究科, 助教 (30625926)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: HIV-2 / 組換え流行株 / 宿主因子 / 293T細胞 / HeLa細胞 / 細胞指向性 / HIV感染抑制因子 / 初代培養細胞

Outline of Final Research Achievements

In vitro replication kinetics of novel HIV-2 circulating recombinant forms in various cell lines as well as in primary cells have been studied. The chemokine receptor CCR5 is used by novel HIV-2 circulating recombinant forms as a coreceptor to enter cells. The findings from this study not only offer academic knowledge of novel HIV-2 circulating recombinant forms, but also help improve treatment for these novel HIV-2 infected patients.

Research Products

• [Journal Article] Structural Insights into HIV-1 Vif-APOBEC3F Interaction (2016)
  • Author(s): Nakashima M, Ode H, Kawamura T, Kitamura S, Naganawa Y, Awazu H, Tsuzuki S, Matsuoka K, Nemoto M, Hachiya A, Sugiura W, Yokomaku Y, Watanabe N, Iwatani Y
  • Journal Title: Journal of Virology Volume: 90 Issue: 2 Pages: 1034-1047
  • DOI: 10.1128/jvi.02369-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] エリートHIVコントローラーの謎 (2014)
  • Author(s): 根本理子
  • Journal Title: 日本生物工学会誌 Volume: 92 Pages: 189-189
• [Presentation] A novel TYK2 compound heterozygous mutation in the patients with primary immunodeficiency (2015)
  • Author(s): 根本理子
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2015-12-02
• [Presentation] Exome sequencing identified a novel tyk2 compound heterozygous mutation in 2 siblings with primary immunodeficiency (2014)
  • Author(s): Michiko Nemoto
  • Organizer: Joint Meeting of the 1st Africa International Biotechnology & Biomedical Conference and the 8th International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: Safari Park Hotel, NAIROBI, KENYA
  • Year and Date: 2014-09-10 – 2014-09-12