| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
We transferred the IL-21 gene into primary NK cells with retroviral vector. The both membrane-binding and secretory type of IL-21 retroviral vectors were constructed. Average transduction efficiencies of IL-21 genes were over 90%. The cytotoxicity of gene-transduced NK cells against K562 and Jurkat cell lines was assessed by four-hour and 72-hour co-culture assay, which revealed augmentation of the cytotoxicity of IL-21 gene transferred NK cells. We confirmed the elevation of IL-21 expression and perforin and granzyme B production in gene-transferred NK cells. That may be one of the reasons of cytotoxicity augmentation. NK cell life span did not extend by both membrane-binding and secretory type of IL-21 gene transduction. While the cytotoxicity of the membrane-binding type would not acheive that of the secretory type, there was no significant difference for the cytotoxicity. The membrane-binding type is expected to reduce cytokine-release symptoms.
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