| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We attempted to generate mesothelin-specific cytotoxic T lymphocytes (CTLs) for developing novel immunotherapy for treating refractory pediatric acute myeloid leukemia. We cultured monocytes from peripheral blood mononuclear cells in a cytokine mix for 1 week so that monocytes could differentiate into dendritic cells. Furthermore, we attempted to generate mesothelin-specific CTLs after stimulating T lymphocytes with mesothelin-peptide-pulsed dendritic cells and a cytokine mix. Although we were unable to effectively generate mesothelin-specific CTLs, we found variable specificity in the generated mesothelin-specific CTLs. We believe our method needs further improvements before it can be applied clinically.
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