Role of Evi5 in the progression of neuroblastoma

• Project/Area Number: 26860802
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Kobe University
• Principal Investigator: Hirase Satoshi 神戸大学, 医学(系)研究科(研究院), 医学研究員 (50622883)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経芽腫 / 自然消退 / Evi5

Outline of Final Research Achievements

While more than 50% of neuroblastoma patients with stage 4 disease have experienced tumor relapses, patients with stage 4S pattern of disease (characterized by metastases limited to liver, skin, and bone marrow) have experienced spontaneous tumor regression. The molecular mechanism responsible for the difference between stage 4 and 4S patinets remains elusive. In the present study, we have focused on Evi5, which was isolated from t(1;10)(p22;q21) in non-tumor cells from a stage 4S patient, and examined its role in the progression of neuroblastoma. We have identified a novel Evi5 isoform (Evi5-NB) that is specifically expressed in neuroblastoma, and revealed that the sphere, colony and xenograft formation of neuroblastoma cells were diminished upon Evi5 knockdown.

Research Products

• [Journal Article] Involvement of aldehyde dehydrogenase 1A2 in the regulation of cancer stem cell properties in neuroblastoma. (2015)
  • Author(s): Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hasegawa D, Kosaka Y, Matsuo M, Hayakawa A, Takeshima Y, Iijima K, Nishio H, and Nishimura N.
  • Journal Title: Int J Oncol Volume: 46 Issue: 3 Pages: 1089-1098
  • DOI: 10.3892/ijo.2014.2801
  • Peer Reviewed
• [Journal Article] Differential expression of minimal residual disease markers in peripheral blood and bone marrow samples from high-risk neuroblastoma patients. (2015)
  • Author(s): Yamamoto N, Kozaki A, Hartomo TB, Yanai T, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Hirase S, Mori T, Hayakawa A, Iijima K, Nishio H, and Nishimura N.
  • Journal Title: Oncol Let Volume: 10 Issue: 5 Pages: 3228-3232
  • DOI: 10.3892/ol.2015.3710
  • Peer Reviewed
• [Presentation] Two high-risk neuroblastoma cases with early detection of tumor relapse/re-growth by consecutive minimal residual disease monitoring. (2015)
  • Author(s): Saito A, Hirase S, Kozaki A, Yanai T, Hasegawa D, Kawasaki K, Kosaka Y, Hayakawa A, Iijima K, Nishio H, and Nishimura N.
  • Organizer: 11th Asian Society for Pediatric Research
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-15
  • Int'l Joint Research
• [Presentation] 神経芽腫がん幹細胞の発生・分化における細胞内小胞輸送の制御因子Rab6Bの役割. (2014)
  • Author(s): 西村範行, Thi Van Huyen Pham, Tri Budi Hartomo, 山本暢之, 平瀬敏志, 早川晶, 矢内友子, 長谷川大一郎, 川崎圭一郎, 小阪嘉之, 松尾雅文, 飯島一誠, 西尾久英.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] Aldehyde dehydrogenase ALDH1A2 regulates cancer stem cell properties in neuroblastoma. (2014)
  • Author(s): Nishimura N, Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hayakawa A, Yanai T, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Iijima K, Nishio H.
  • Organizer: 第56回日本小児血液・がん学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-11-28 – 2014-11-30
• [Presentation] Aldehyde dehydrogenase 1A2 expression correlates with cancer stem cell properties in neuroblastoma. (2014)
  • Author(s): Nishimura N, Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hayakawa A, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Takeshima Y, Iijima K, and Nishio H.
  • Organizer: Advances in Neuroblastoma Research Conference 2014
  • Place of Presentation: Cologne, Germany
  • Year and Date: 2014-05-13 – 2014-05-16
• [Presentation] Induction of ALDH1A2 expression is critical for cancer stem cell properties in neuroblastoma. (2014)
  • Author(s): Nishimura N, Hartomo TB, Pham TVH, Yamamoto N, Hirase S, Hayakawa A, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Takeshima Y, Iijima K, and Nishio H.
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2014-04-05 – 2014-04-09