| Project/Area Number |
26860809
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Yamaguchi University (2015)
Kyushu University (2014) |
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Principal Investigator |
KUSUDA Takeshi 山口大学, 医学部附属病院, 講師 (90710533)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 川崎病 / バイオフィルム / 偽結核菌 / セレウス菌 / 枯草菌 / 質量分析計 |
|---|
| Outline of Final Research Achievements |
KD samples elicited proinflammatory cytokine responses from human coronary artery endothelial cells(HCAECs). By LC-MS analysis of KD serum samples collected at 3 different periods,we detected a variety of KD-specific molecules in the lipophilic fractions. Serum KD-specific molecules showed m/z and MS/MS fragmentation patterns almost identical to those of MAMPs obtained from the biofilms formed in vitro (common MAMPs from Bacillus cereus, Yersinia pseudotuberculosis and Staphylococcus aureus) at the 1st study period,and from the biofilms formed in vivo (common MAMPs from Bacillus cereus, Bacillus subtilis/Bacillus cereus/Yersinia pseudotuberculosis and Staphylococcus aureus) at the 2nd and 3rd periods. The biofilm extracts from Bacillus cereus, Bacillus subtilis, Yersinia pseudotuberculosis and Staphylococcus aureus also induced proinflammatory cytokines by HCAECs. By the experiments with IgG affinity chromatography,some of these serum KD-specific molecules bound to IgG.
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