| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We found that morphological changes in non-neuronal organs observed in male deficient MeCP2 gene mice, Rett syndrome (RTT) model mouse, are due to abnormal secretion of certain physiologically active substances. In the cultured cells that MeCP2 gene knockdown cells using RNAi and reproduced the results seen in vivo by biochemical analysis. The genes involved in the biosynthesis of this substance were analyzed by quantitative PCR and we could extract the expression variable gene. Analysis by quantitative PCR must be performed comprehensively and it was not possible to collect sufficient data for analysis of female mice deficient in MeCP 2 gene which is a true RTT model mouse which was the final target.
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