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In vitro analysis of the mechanisms of intravenous immunoglobulin and prednisolone for the prevention of coronary artery abnormalities in Kawasaki disease.

Research Project

Project/Area Number 26860814
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatrics
Research InstitutionKagoshima University

Principal Investigator

Ueno Kentaro  鹿児島大学, 医歯学域医学部・歯学部附属病院, 助教 (20644892)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords川崎病 / 血管炎 / 細胞傷害 / 細胞生存 / アポトーシス / 免疫グロブリン / ステロイド
Outline of Final Research Achievements

We aimed to evaluate the pathogenic role of cell survival and death in in vitro model of Kawasaki disease (KD) vasculitis. KD patients (42) and febrile patients (10 as controls) were analyzed. HUVECs were stimulated with sera from patients with KD were left untreated or treated with immunoglobulin (IG) and/or prednisolone (PSL). Loss of viability in patients with KD were observed compared to controls (P<0.001). Relative levels of cytotoxicity, caspase-3/7 activity and HMGB-1 were significantly higher in KD compared to controls (P<0.001, respectively), while significant decrease in phosphorylated Akt/Akt ratio was observed(P=0.023). The increased cytotoxic effects and decreased phosphorylated p-Akt/Akt ratio were closely associated with CAA (P = 0.002 and P = 0.032, respectively). Furthermore treatment with IG significantly reduced the relative levels of cytotoxicity, caspase 3/7 activity and increased levels of p-Akt/Akt ratio on cultured HUVECS in KD (P < 0.001, respectively).

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results)

  • [Journal Article] Circulating Platelet-Neutrophil Aggregates Play a Significant Role in Kawasaki Disease2015

    • Author(s)
      Ueno K, Nomura Y, Morita Y, Eguchi T, Masuda K, Kawano Y.
    • Journal Title

      Circulation Journal

      Volume: 79 Issue: 6 Pages: 1349-1356

    • DOI

      10.1253/circj.CJ-14-1323

    • NAID

      130005073158

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circulating Platelet-Neutrophil Aggregates Play a Significant Role in Kawasaki Disease.2015

    • Author(s)
      Kentaro Ueno, Yuichi Nomura, Yasuko Morita, Taisuke Eguchi, Kiminori Masuda , Yoshifumi Kawano.
    • Journal Title

      Circulation Journal

      Volume: 未定

    • NAID

      130005073158

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 川崎病血管炎における内皮細胞の生存・障害と冠動脈後遺症発症との関連性の検討2015

    • Author(s)
      上野 健太郎
    • Organizer
      第35回日本川崎病学会・学術集会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Circulating Platelet-Neutrophil Aggregates play a significant role in Kawasaki Disease.2015

    • Author(s)
      Kentaro Ueno
    • Organizer
      Eleventh International Kawasaki Disease Symposium 2015
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2015-02-03 – 2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 川崎病血管炎における血小板好中球複合体の関与の検討.2014

    • Author(s)
      上野 健太郎
    • Organizer
      第50回日本小児循環器学会総会・学術集会
    • Place of Presentation
      岡山コンベンションセンター、岡山
    • Year and Date
      2014-07-03 – 2014-07-05
    • Related Report
      2014 Research-status Report
  • [Presentation] In vitro analysis of the mechanisms of intravenous immunoglobulin and prednisolone for the prevention of coronary artery abnormalities in Kawasaki disease.2014

    • Author(s)
      Kentaro Ueno
    • Organizer
      48th Annual Meeting of the Association for European Paediatric and Congenital Cardiology.
    • Place of Presentation
      Helsinki, Finland
    • Year and Date
      2014-05-21 – 2014-05-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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