Clarification of SMN expression regulation mechanism using SMA patient-derived cells
| Project/Area Number |
26860829
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Arakawa Reiko 東京女子医科大学, 医学部, 講師 (40623111)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脊髄性筋萎縮症 / SMNタンパク質 / ＳＭＮタンパク質 / イメージングフローサイトメトリー |
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| Outline of Final Research Achievements |
Spinal muscular atrophy is a neurodegenerative disorder caused by the deficient expression of survival motor neuron protein in motor neurons. In SMA patients, SMN protein derived from SMN2 gene plays an important role. In this study, we developed the imaging flow cytometry method having both functions of fluorescence microscope system and flow cytometry method as an analysis method for SMN protein. Using this method, we revealed the expression patterns and subcellular localization of SMN protein in SMA patient-derived fibroblasts and lymphoblasts.
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Report
(4 results)
Research Products
(10 results)
