miRNA profiling in serum exosome of premature infant with bronchopulmonary displaisia and mouse lung exposed to hyperoxia.

• Project/Area Number: 26860850
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Fukushima Medical University
• Principal Investigator: Go Hayato 福島県立医科大学, 医学部, 助教 (30443857)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: microRNA / miR-21 / 新生児慢性肺疾患 / miRNA / エクソソーム / 高濃度酸素暴露 / Exosome

Outline of Final Research Achievements

In this study, we performed miRNA profiling to validate identified miRNAs as potential biomarkers for BPD (bronchopulmonary displaisia) using serum exosome in premature infants and neonatal mouse lung exposed to hyperoxia. In human miRNA array, we identified 45 miRNAs were universally expressed in BPD patients and non BPD patients. miR-21 was upregulatd in BPD patients on DOL28.
In BPD mouse model, 6 miRNAs were upregulated and 4 miRNA were downregilated. miR-21 was detected as common miRNA that changed among BPD patients and BPD mouse model.

Research Products

• [Journal Article] MiR-196a regulates heme oxygenase-1 by silencing Bach1 in the neonatal mouse lung. (2016)
  • Author(s): Go H, La P, Namba F, Ito M, Yang G, Brydun A, Igarashi K, Dennery PA.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 311 Issue: 2 Pages: L400-L411
  • DOI: 10.1152/ajplung.00428.2015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plasma TGF-<i>β</i>1 Levels Are Elevated in Down Syndrome Infants with Transient Abnormal Myelopoiesis (2016)
  • Author(s): Maeda H, Go H, Imamura T, Sato M, Momoi N, Hosoya M.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 240 Issue: 1 Pages: 1-5
  • DOI: 10.1620/tjem.240.1
  • NAID: 130005169156
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed / Open Access
• [Journal Article] Early Postnatal Seizures in a Neonate with Wolf-Hirschhorn Syndrome. (2016)
  • Author(s): Go H, Haneda K, Maeda H, Ogasawara K, Imamura T, Momoi N, Hosoya M.
  • Journal Title: AJP Rep. Volume: 6
  • Peer Reviewed / Open Access
• [Presentation] miRNA profiling in serum exosome of premature infant with bronchopulmonary displaisia and mouse lung exposed to hyperoxia. (2017)
  • Author(s): Hayato Go
  • Organizer: PAS meeting
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2017-05-05
  • Int'l Joint Research
• [Presentation] 慢性肺疾患患児における血清中エクソソームmiRNAプロファイリング (2016)
  • Author(s): 郷勇人
  • Organizer: 日本周産期新生児学会
  • Place of Presentation: 富山
  • Year and Date: 2016-07-16