Cell Sheet Transplantation in Utero: Using a Fetal Rats to Approach New Fetal Therapy

• Project/Area Number: 26860855
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Research Institute of Shimizu Construction Co. (2016); Tokyo Women's Medical University (2014-2015)
• Principal Investigator: Kakimoto Takashi 清水建設株式会社技術研究所, その他部局等, 研究員 (70566687)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 再生医療 / 胎児期治療 / 細胞シート / 胎児治療 / 細胞シート工学

Outline of Final Research Achievements

Summary　We have developed cell sheet (CS) based regenerative medicine and successfully applied it to clinical settings for reconstructing various tissues. In this study, we present the possibility of CS transplantation for fetal therapy. Methods Allogenic epithelial cell sheets were fabricated from oral mucosal epithelial cells isolated from adult GFP transgenic rats and cultivated on temperature-responsive culture inserts, transplanted to the ulcer sites of each rats’ fetus (E17) in utero. Three days later, fetuses were sacrificed to examine macroscopically and histologically. Results　The transplanted CS sealed up the ulcer, while skin damage was only observed in sham controls. Immunohistochemical observations revealed that transplanted CSs were stably adhered without suture or sealing and epidermal regeneration occurred at the transplanted sites. Conclusion　Our results suggest cell sheet in utero transplantation to fetus is possible, which is applicable to fetal therapy.

Research Products

• [Presentation] 細胞シートを用いた二分脊椎症胎児期治療 (2016)
  • Author(s): 柿本隆志,藏本吾郎,大和雅之,岡野光夫
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2016-03-16
• [Presentation] 脊髄髄膜留に対する細胞シート移植 (2015)
  • Author(s): 柿本隆志,藏本吾郎,大和雅之,岡野光夫
  • Organizer: 第１３回日本胎児治療学会学術集会
  • Place of Presentation: 横浜情報文化センター（神奈川県横浜市）
  • Year and Date: 2015-11-21
• [Presentation] 細胞シートを用いた胎児期治療 (2015)
  • Author(s): 柿本隆志、藏本吾郎、大和雅之、岡野光夫
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 細胞シートを用いた胎児期治療 (2014)
  • Author(s): 柿本隆志、藏本吾郎、大和雅之、岡野光夫
  • Organizer: 第12回日本胎児治療学会
  • Place of Presentation: 福岡県久留米市久留米大学病院
  • Year and Date: 2014-11-29 – 2014-11-30