Function of sling associated molecules in deep venous thrombosis

• Project/Area Number: 26860869
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: The University of Tokyo
• Principal Investigator: Kuwano Yoshihiro 東京大学, 医学部附属病院, 登録研究員 (60625044)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 好中球 / シグナル伝達 / Gai subunit / 深部静脈血栓症

Outline of Final Research Achievements

Neutrophils arrest in Gai2 deficient neutrophils is defective. Gai3 deficient neutrophils showed reduced transmigration but normal arrest. Gai3, but not Gai2, deficient neutrophils showed significantly reduced migration and directionality also in a microfluidic device. This suggest that the activation of Gai2 subunit or the inhibition of Gai3 subunit in deep venous thrombosis may cause the inhibition of transmigration of neutrophils from the venous wall and the exacerbation of the disease activity. The specific control of the activity of these molecules may lead to the improvement of the symptom in deep venous thrombosis with less side effects.

Research Products

• [Int'l Joint Research] ラホヤアレルギー免疫研究所(米国)
• [Journal Article] Gαi2 and Gαi3 Differentially Regulate Arrest from Flow and Chemotaxis in Mouse Neutrophils. (2016)
  • Author(s): Kuwano Y, Adler M, Zhang H, Groisman A, Ley K.
  • Journal Title: J Immunol Volume: Epub
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association of anti-RNA polymerase III antibody and malignancy in Japanese patients with systemic sclerosis. (2015)
  • Author(s): Saigusa R, Asano Y, Nakamura K, Miura S, Ichimura Y, Takahashi T, Toyama T, Taniguchi T, Noda S, Aozasa N, Akamata K, Sumida H, Miyazaki M, Tamaki Z, Yanaba K, Kuwano Y, Sato S.
  • Journal Title: J Dermatol Volume: 42 Issue: 5 Pages: 524-7
  • DOI: 10.1111/1346-8138.12827
  • Peer Reviewed
• [Journal Article] Successful experience of rituximab therapy for systemic sclerosis-associated interstitial lung disease with concomitant systemic lupus erythematosus. (2014)
  • Author(s): Sumida H, Asano Y, Tamaki Z, Aozasa N, Taniguchi T, Takahashi T, Toyama T, Ichimura Y, Noda S, Akamata K, Miyazaki M, Kuwano Y, Yanaba K, Sato S.
  • Journal Title: J Dermatol Volume: 41 Issue: 5 Pages: 418-20
  • DOI: 10.1111/1346-8138.12461
  • Peer Reviewed