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Identification and characterization of endothelium-derived factors that promote melanoma stem cells

Research Project

Project/Area Number 26860883
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionEhime University

Principal Investigator

Shiraiahi Ken  愛媛大学, 医学部附属病院, 講師 (80710863)

Co-Investigator(Renkei-kenkyūsha) Murakami Masamoto  愛媛大学, 医学部付属病院, 講師 (20278302)
Hanakawa Yasushi  愛媛大学, 医学部付属病院, 講師 (90284398)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsメラノーマ / 癌幹細胞 / リンパ管内皮 / ニッチ / 内皮細胞
Outline of Final Research Achievements

Co-culture of lymphatic endothelial cells (LECs) with melanoma stem cells revealed that LECs promoted the self-renewal of melanoma stem cells, as evidenced by increased sphere formation capacity. Using RT-PCR-based transcriptional profiling, we found that the chemokines CXCL9, CXCL10 and CXCL11 were strongly expressed by LECs when co-cultured with CSCs. Addition of recombinant CXCL10 or CXCL11 promoted sphere formation of melanoma stem cells, and sphere formation was significantly inhibited by a CXCR3 neutralizing antibody. The expression of CXCR3 was strongly increased in sphere-forming melanoma cells compared to melanoma cells in adherent cultures and that CXCR3 is also expressed by a subpopulation of tumor cells in human malignant melanomas of the skin. Together, these findings indicate an important role of CXCR3 activation in the maintenance of CSCs and suggest that CXCR3 blockade might represent a novel therapeutic strategy to specifically target melanoma stem cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Cell motion predicts human epidermal stemness2015

    • Author(s)
      Nanba D, Toki F, Tate S, Imai M, Matsushita N, Shiraishi K, Sayama K, Toki H, HigashiyamaS, and Barrandon Y.
    • Journal Title

      The Journal of Cell Biology(JCB)

      Volume: 209 Pages: 305315-305315

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 癌幹細胞を標的とした新たな癌治療戦略2014

    • Author(s)
      白石研
    • Organizer
      e-see研究会
    • Place of Presentation
      松山市
    • Year and Date
      2014-10-11
    • Related Report
      2014 Research-status Report
  • [Presentation] メラノーマ幹細胞をプロモートする新たな内皮細胞由来因子の同定2014

    • Author(s)
      白石研
    • Organizer
      四国四大学皮膚科研究会
    • Place of Presentation
      徳島市
    • Year and Date
      2014-06-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification and characterization of Lymphatic endothelium-derived factors that promote cancer stem cells2014

    • Author(s)
      白石研
    • Organizer
      第38回 日本リンパ管学会総会
    • Place of Presentation
      北里大白金キャンパス
    • Year and Date
      2014-06-20 – 2014-06-21
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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