| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Merkel cell carcinoma (MCC) is a highly aggressive skin cancer linked to a contributory virus, Merkel cell polyomavirus (MCPyV). MCPyV DNA has been confirmed to be present in approximately 80% of MCCs. We had several extremely rare cases of MCC combined with Squamous cell carcinoma (SCC). All reported MCC combined with SCC were MCPyV-negative. Thus, we hypothesized that there are differences of the cell characteristics between MCPyV-positive and -negative MCCs in a coexistence environment with SCC. SCC cell line, DJM-1, inhibits proliferation of MCPyV-positive MCC cell line, MKL-1 cells, but not MCPyV-negative cell lines in collagen gel matrix culture. DJM-1 cells induce apoptosis of MKL-1 cells. MKL-1 cells inhibit proliferation of DJM-1 cells. This culture system shows the difference of biological behavior between MCPyV-positive MCC and MCPyV-negative MCC. It remains possible that MCPyV-positive and -negative MCC may develop through different tumorigenic pathways.
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