Study on mechanisms of cutaneous amyloid fibril formating
| Project/Area Number |
26860885
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
MINE Yoshiko 長崎大学, 病院(医学系), 助教 (90381235)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | アミロイド / ケラチン / 弾性線維 |
|---|
| Outline of Final Research Achievements |
Amyloidosis is a group of disease that destroys cells or tissues by accumulating insoluble materials with beta-sheet structure in cells. The precise mechanisms are not fully understood yet. Using cutaneous amyloidosis, we immunohistochemically demonstrated that cytokeratin(CK) 5 was collocated with amyloid fibrils (AF) in the dermis. We succeeded to show that one of synthetic peptides covering c-terminus of CK5 could form AF. Furthermore, we found a member of extracellular matrix (ECM-X) codistributed with AF in the dermis. By in vitro AF assay with ThT, we demonstrated that recombinant ECM-X accelerated F formation of CK5-derived peptide. Taken these, cutaneous AF formation proceeds with a help of special bystander such as ECM-X. The results of this unique points of AF formation may help drug development to amyloidosis in any organs.
|
Report
(3 results)
Research Products
(3 results)
