| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Amyloidosis is a group of disease that destroys cells or tissues by accumulating insoluble materials with beta-sheet structure in cells. The precise mechanisms are not fully understood yet. Using cutaneous amyloidosis, we immunohistochemically demonstrated that cytokeratin(CK) 5 was collocated with amyloid fibrils (AF) in the dermis. We succeeded to show that one of synthetic peptides covering c-terminus of CK5 could form AF. Furthermore, we found a member of extracellular matrix (ECM-X) codistributed with AF in the dermis. By in vitro AF assay with ThT, we demonstrated that recombinant ECM-X accelerated F formation of CK5-derived peptide. Taken these, cutaneous AF formation proceeds with a help of special bystander such as ECM-X. The results of this unique points of AF formation may help drug development to amyloidosis in any organs.
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