Significance of KEAP1 mutation in melnaoma cells.

Research Project

Project/Area Number	26860891
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Dermatology
Research Institution	Iwate Medical University
Principal Investigator	Miura Shinpei 岩手医科大学, 医学部, 助教 (30713226)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	KEAP1 / NRF2 / CDDP / ドライバー変異 / 悪性黒色腫 / EMT / 薬剤耐性
Outline of Final Research Achievements	We identified KEAP1 inactivation mutation in booth malignant melanoma cell lines and primary tumors. Mutations of the KEAP1 genes related to drug resistance （CDDP and dacarbazine）. The ratio of reduced-to-oxidized glutathione (GSH/GSSG) was decreased by treatment with CDDP or DTIC in siNRF2-transfected cells. However, the mutation did not confer the tumorigenicty for xenografts. Theses results suggest that EAP/NRF2 inactivation could not be a driver mutation in melanoma cells.

Report

(3 results)