• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Significance of KEAP1 mutation in melnaoma cells.

Research Project

Project/Area Number 26860891
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionIwate Medical University

Principal Investigator

Miura Shinpei  岩手医科大学, 医学部, 助教 (30713226)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsKEAP1 / NRF2 / CDDP / ドライバー変異 / 悪性黒色腫 / EMT / 薬剤耐性
Outline of Final Research Achievements

We identified KEAP1 inactivation mutation in booth malignant melanoma cell lines and primary tumors. Mutations of the KEAP1 genes related to drug resistance (CDDP and dacarbazine). The ratio of reduced-to-oxidized glutathione (GSH/GSSG) was decreased by treatment with CDDP or DTIC in siNRF2-transfected cells. However, the mutation did not confer the tumorigenicty for xenografts. Theses results suggest that EAP/NRF2 inactivation could not be a driver mutation in melanoma cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.2014

    • Author(s)
      Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, Maeda F, Takahashi K, Akasaka T, Masuda T. Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.
    • Journal Title

      Am J Dermatopathol

      Volume: 36(3) Issue: 3 Pages: 211-216

    • DOI

      10.1097/dad.0b013e3182964e02

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi