| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The purpose of the present study was to observe changes in autophagy reaction system factors under skin induction using skin grafts from Atg7-knockout mice and explore the possibility of new agents for treatment of inflammation from the autophagy reaction system. To that end, we observed mature skin tissue in SCID mice transplanted with skin grafts from Atg7-knockout mice. And then analyzed various skin disease tissues under special staining using autophagy-related factors.
The results showed decreases in filaggrin, loricrin, and involucrin as well as hair abnormalities in the skin into which the Atg7-knockout mouse skin was transplanted. Furthermore, the cathepsin family, which is a group of proteolytic enzymes in lysosomes, was also immunostained in psoriasis, and enhanced staining was also confirmed with cathepsinD and cathepsinL staining. These results suggest that the autophagy reaction system may be involved in inflammatory skin diseases.
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