Function analysis of autophagy in a skin inflammation aiming at new treatment
Project/Area Number |
26860897
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | Juntendo University |
Principal Investigator |
Takagi Atsushi 順天堂大学, 医学部, 非常勤講師 (40459160)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 皮膚角化 / オートファジー / Atg7 / Atg7 / LC3 |
Outline of Final Research Achievements |
The purpose of the present study was to observe changes in autophagy reaction system factors under skin induction using skin grafts from Atg7-knockout mice and explore the possibility of new agents for treatment of inflammation from the autophagy reaction system. To that end, we observed mature skin tissue in SCID mice transplanted with skin grafts from Atg7-knockout mice. And then analyzed various skin disease tissues under special staining using autophagy-related factors. The results showed decreases in filaggrin, loricrin, and involucrin as well as hair abnormalities in the skin into which the Atg7-knockout mouse skin was transplanted. Furthermore, the cathepsin family, which is a group of proteolytic enzymes in lysosomes, was also immunostained in psoriasis, and enhanced staining was also confirmed with cathepsinD and cathepsinL staining. These results suggest that the autophagy reaction system may be involved in inflammatory skin diseases.
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Report
(4 results)
Research Products
(1 results)
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[Journal Article] The significant role of autophagy in the granular layer in normal skin differentiation and hair growth.2014
Author(s)
Yoshihara, N., Ueno, T., Takagi, A., Trejo, JA., Haruna, K., Suga, Y., Komatsu, M., Tanaka, K., *Ikeda, S.
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Journal Title
Arch. Dermatol. Res.
Volume: 307
Issue: 2
Pages: 159-169
DOI
Related Report
Peer Reviewed / Open Access