| Project/Area Number |
26860905
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Kyushu University (2015)
Kurume University (2014) |
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Principal Investigator |
NISHIKAWA Ryuhei 九州大学, (連合)農学研究科(研究院), 学術研究員 (30534531)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Early endosome antigen 1 / EEA1 / 天疱瘡 / 自己免疫疾患 / BIOCHIP / auto-antibody / pemphigus disease / autoimmune disease / EEA-1 |
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| Outline of Final Research Achievements |
The major antigens of typical pemphigus disease are desmoglein, desmocollins and a plakin proteins. However, there are novel antigen supposed to be studied to classify the pemphigus disease patients. We used immunoblotting using a serum from an atypical autoimmune bullous disease patient to detect autoantibody, which react with unknown 175 kDa protein. Subsequent studies using two-dimensional gel electrophoresis, immunoblotting and mass-spectrometry identified 175 kDa protein as early endosome antigen 1 (EEA1). This finding was confirmed by subsequent immunological studies, including immunofluorescence of skin and cultured keratinocyte and two-dimensional gel immunoblotting with anti-EEA1 monoclonal antibody and absorption studies using EEA1 recombinant protein. We also developed a novel BIOCHIP assay using full length EEA1 cDNA to detect anti-EEA1 antibodies. However, none of 35 pemphigus sera showed anti-EEA1 antibodies in the BIOCHIP assay except the serum from index case patient.
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