| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Previously, we found thrombospondin-4 (TSP4) expressed highly in atherosclerotic lesions of mouse aorta. Thus, we developed anti-TSP4 monoclonal IgG radio-labelled with 99mTc (99mTc-HTA). 99mTc-HTA showed higher accumulation in atherosclerotic aortas of mice; however, we also found that the non-targeted monoclonal IgG radio-labelled with 99mTc (99mTc-HNA) showed similar distribution. Therefore, we next focused on the relationship between radio-labelled IgG accumulation and macrophage polarization. 99mTc-HNA showed higher accumulation in M1 macrophage than M2 and M0 macrophage. The expression levels of Fc gamma receptor I and II were also higher in M1 macrophage than M2 and M0 macrophage. These results suggest that radio-labelled monoclonal IgG can visualize the active inflammation in atherosclerotic plaque formation independent of its target biomolecules.
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