Elucidation of radioresistance mechanisms in cancer stem cells focusing on mTOR activation under hypoxic/nutrient starvation conditions
| Project/Area Number |
26860974
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | mTOR / 低酸素状態 / 低栄養状態 / 放射線抵抗性 / 癌肝細胞 / PLDR / 癌幹細胞 / 低栄養 / 低酸素 |
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| Outline of Final Research Achievements |
The presence of radiation resistant cells in solid human tumors is one of the most important factors for radiotherapy. In solid human tumors, it is well known that hypoxia causes cellular radioresistance and that tumor hypoxia is consistently associated with nutrient starvation conditions. To identify molecular targets for selective regulation of radioresistance under hypoxic/nutrient starvation conditions, mTOR, known as nutrient sensor, was focused. Under nutrient starvation conditions, mTOR activity is thought to be inhibited and cells show radioresistance. Liver cancer cell lines, however, showed that mTOR activation and increased radiosensitivity under nutrient starvation conditions. Inhibition of mTOR expression by siRNA suppresses the increased radiosensitivity under nutrient starvation conditions. This study demonstrates for the first time that nutrient starvation conditions activate mTOR and increase radiosensitivity through mTOR activation in liver cancer cell lines.
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Report
(4 results)
Research Products
(11 results)
