Relationship of ceraide in cellular response of heavy ion irradiation
| Project/Area Number |
26860977
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | セラミド / バイスタンダー効果 |
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| Outline of Final Research Achievements |
X-ray irradiation was applied to fibroblast cell line and chondrosarcoma cell line and co-cultured the unirradiated cells with irradiated cells to analyze cell response to radiation. In particular, in order to analyze the involvement of ceramide in cellular response of co-cultured non-irradiated cells, DNA damage and quantitative measurement of ceramide were carried out by image analysis using fluorescent immunostaining method. Radiation response of non-irradiated cells (bystander effect) was confirmed and fundamental studies were conducted to investigate the influence of ceramide.
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Report
(4 results)
Research Products
(1 results)
