The role of the microRNA in the radiation resistance using pancreatic cancer cell
| Project/Area Number |
26860978
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 放射線抵抗性 / 膵癌 / microRNA / 放射線治療 / 重粒子 / 炭素イオン線 / Gemcitabine / 化学放射線治療 |
|---|
| Outline of Final Research Achievements |
First of all, we made a cell line that show radiation resitance by repeating low dose Gemcitabine exposure. We use a pancreatic cancer cell line with name of Panc-1. We name the radiation resistance cell line as Panc-1 XR. We compair the microRNA status of Panc-1 XR and wild type. Then try temporaly insertion of some kind of microRNA. Finally we made a cell line that stably overexpress the mir-34a by transecting vector. The stable OE cell line (panc-1-34aOE) show radiation sensibility against X-ray irradiation. Therefore, approaching to the microRNA mir-34a could improve the resistance against treatment in the pancreatic cancer.
|
Report
(4 results)
Research Products
(3 results)
