| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
First of all, we made a cell line that show radiation resitance by repeating low dose Gemcitabine exposure. We use a pancreatic cancer cell line with name of Panc-1. We name the radiation resistance cell line as Panc-1 XR. We compair the microRNA status of Panc-1 XR and wild type. Then try temporaly insertion of some kind of microRNA. Finally we made a cell line that stably overexpress the mir-34a by transecting vector. The stable OE cell line (panc-1-34aOE) show radiation sensibility against X-ray irradiation. Therefore, approaching to the microRNA mir-34a could improve the resistance against treatment in the pancreatic cancer.
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