Relationship between inhibition of glutamate-glutamine cycle and 14C-Acetate uptake in rat brain

Research Project

Project/Area Number	26860992
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Radiation science
Research Institution	Osaka University
Principal Investigator	YANAMOTO Kazuhiko 大阪大学, 医学系研究科, 助教 (70531531)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords	酢酸 / グリア細胞 / グルタミン酸ーグルタミンサイクル / 神経細胞
Outline of Final Research Achievements	The brain uptake of 14C-acetate in the dysfunction of glutamate-glutamine (Glu-Gln) cycle between neuron and glia was measured. In the neuronal or glial cells-lesioned rat model, the uptake of 14C-acetate was decreased in the lesioned area. Furthermore, the 14C-acetate uptake was also reduced in the dihydrokainate (an inhibitor of glutamate transporter-1)-infused rat brain. These results suggest that the 14C-acetate uptake into glial cells might be affected by the process of uptake of glutamate into glial cells.