Chronic effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model
Project/Area Number |
26861028
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Radiation science
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Research Institution | National Institute of Radiological Sciences |
Principal Investigator |
Takuwa Hiroyuki 国立研究開発法人放射線医学総合研究所, 分子イメージング研究センター, 研究員 (40508347)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 慢性低灌流 / 脳卒中 / モデル動物 / 二光子励起レーザー顕微鏡 / 小動物PET / PET / 二光子顕微鏡 / 病態モデルマウス / 脳血流 / 神経機能障害 / 神経細胞脱落 / 脳循環代謝障害 / 11C]flumazenil-PET |
Outline of Final Research Achievements |
In this study, we investigated the effects of chronic cerebral hypoperfusion on brain induced by a unilateral common carotid artery occlusion (UCCAO). As the first step of this study, we developed a misery perfusion mouse model using UCCAO and measured autoregulatory vasodilation with two-photon microscopy. The diameter of arterioles and capillaries during the resting state were increased 7 days after UCCAO. After UCCAO, autoregulatory vasodilation of arterioles during normocapnia was negatively correlated with a vasodilation during hypercapnia. The second step of this study was to investigate the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia. Neuronal density evaluated 28 days after UCCAO using [11C]flumazenil-PET and histology indicated no neurologic deficit in brain. Functional hyperemia induced by sensory stimulation was assessed using laser-Doppler flowmetry and became worse 28 days after UCCAO.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model.2016
Author(s)
Nishino A, Tajima Y, Takuwa H, Masamoto K, Taniguchi J, Wakizaka H, Kokuryo D, Urushihata T, Aoki I, Kanno I, Tomita Y, Suzuki N, Ikoma Y, Ito H.
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Journal Title
Scientific reports
Volume: 27
Issue: 1
Pages: 25072-25072
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy.2014
Author(s)
Tajima Y, Takuwa H, Kokuryo D, Kawaguchi H, Seki C, Masamoto K, Ikoma Y, Taniguchi J, Aoki I, Tomita Y, Suzuki N, Kanno I, Saeki N, Ito H.
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Journal Title
J Cereb Blood Flow Metab.
Volume: 34
Issue: 8
Pages: 1363-1372
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pial arteries respond earlier than penetrating arterioles to neural activation in the somatosensory cortex in awake mice exposed to chronic hypoxia: an additional mechanism to proximal integration signaling?2014
Author(s)
Sekiguchi Y, Takuwa H, Kawaguchi H, Kikuchi T, Okada E, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K, Masamoto K.
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Journal Title
J Cereb Blood Flow Metab.
Volume: 34
Issue: 11
Pages: 1761-1770
DOI
Related Report
Peer Reviewed / Open Access
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