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Chronic effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

Research Project

Project/Area Number 26861028
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

Takuwa Hiroyuki  国立研究開発法人放射線医学総合研究所, 分子イメージング研究センター, 研究員 (40508347)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords慢性低灌流 / 脳卒中 / モデル動物 / 二光子励起レーザー顕微鏡 / 小動物PET / PET / 二光子顕微鏡 / 病態モデルマウス / 脳血流 / 神経機能障害 / 神経細胞脱落 / 脳循環代謝障害 / 11C]flumazenil-PET
Outline of Final Research Achievements

In this study, we investigated the effects of chronic cerebral hypoperfusion on brain induced by a unilateral common carotid artery occlusion (UCCAO). As the first step of this study, we developed a misery perfusion mouse model using UCCAO and measured autoregulatory vasodilation with two-photon microscopy. The diameter of arterioles and capillaries during the resting state were increased 7 days after UCCAO. After UCCAO, autoregulatory vasodilation of arterioles during normocapnia was negatively correlated with a vasodilation during hypercapnia. The second step of this study was to investigate the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia. Neuronal density evaluated 28 days after UCCAO using [11C]flumazenil-PET and histology indicated no neurologic deficit in brain. Functional hyperemia induced by sensory stimulation was assessed using laser-Doppler flowmetry and became worse 28 days after UCCAO.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model.2016

    • Author(s)
      Nishino A, Tajima Y, Takuwa H, Masamoto K, Taniguchi J, Wakizaka H, Kokuryo D, Urushihata T, Aoki I, Kanno I, Tomita Y, Suzuki N, Ikoma Y, Ito H.
    • Journal Title

      Scientific reports

      Volume: 27 Issue: 1 Pages: 25072-25072

    • DOI

      10.1038/srep25072

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dynamic flow velocity mapping from fluorescent dye transit times in the brain surface microcirculation of anesthetized rats and mice.2016

    • Author(s)
      Hoshikawa R, Kawaguchi H, Takuwa H, Ikoma Y, Tomita Y, Unekawa M, Suzuki N, Kanno I, Masamoto K.
    • Journal Title

      Microcirculation

      Volume: 26 Issue: 6 Pages: 416-425

    • DOI

      10.1111/micc.12285

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vasodilation Mechanism of Cerebral Microvessels Induced by Neural Activation under High Baseline Cerebral Blood Flow Level Results from Hypercapnia in Awake Mice.2015

    • Author(s)
      Nishino A, Takuwa H, Urushihata T, Ito H, Ikoma Y, Matsuura T.
    • Journal Title

      Microcirculation

      Volume: 22 Pages: 744-752

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] [11C]Raclopride binding in the striatum of minimally restrained and free-walking awake mice in a positron emission tomography study2015

    • Author(s)
      Takuwa H, Maeda J, Ikoma Y, Tokunaga M, Wakizaka H, Uchida S, Kanno I, Taniguchi J, Ito H, Higuchi M
    • Journal Title

      Synapse

      Volume: 69 Issue: 12 Pages: 600-606

    • DOI

      10.1002/syn.21864

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice2014

    • Author(s)
      Takuwa H, Matsuura T, Nishino A, Sakata K, Tajima Y, Ito H
    • Journal Title

      Journal of neuroscience methods

      Volume: 30 Pages: 9-15

    • DOI

      10.1016/j.jneumeth.2014.08.022

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes in cortical microvasculature during misery perfusion measured by two-photon laser scanning microscopy.2014

    • Author(s)
      Tajima Y, Takuwa H, Kokuryo D, Kawaguchi H, Seki C, Masamoto K, Ikoma Y, Taniguchi J, Aoki I, Tomita Y, Suzuki N, Kanno I, Saeki N, Ito H.
    • Journal Title

      J Cereb Blood Flow Metab.

      Volume: 34 Issue: 8 Pages: 1363-1372

    • DOI

      10.1038/jcbfm.2014.91

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pial arteries respond earlier than penetrating arterioles to neural activation in the somatosensory cortex in awake mice exposed to chronic hypoxia: an additional mechanism to proximal integration signaling?2014

    • Author(s)
      Sekiguchi Y, Takuwa H, Kawaguchi H, Kikuchi T, Okada E, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K, Masamoto K.
    • Journal Title

      J Cereb Blood Flow Metab.

      Volume: 34 Issue: 11 Pages: 1761-1770

    • DOI

      10.1038/jcbfm.2014.140

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] PET技術と生体光イメージング技術の融合と脳疾患研究2016

    • Author(s)
      田桑弘之
    • Organizer
      JAEA放射光科学シンポジウム2016
    • Place of Presentation
      兵庫県 播磨
    • Year and Date
      2016-02-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] フラビン蛍光イメージングへの血流の影響の補正法の検討2015

    • Author(s)
      漆畑拓弥
    • Organizer
      ニューロフォトニクス
    • Place of Presentation
      東京
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] DIAMETER CHANGES TO CEREBRAL BLOOD VESSELS DURING NEURAL ACTIVATION AND DEACTIVATION MEASURED BY TWO-PHOTON MICROSCOPY IN AWAKE MICE2015

    • Author(s)
      田桑弘之
    • Organizer
      XXVIIth International Symposium on Cerebral Blood Flow, Metabolism and Function & XIIth International Conference
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 覚醒マウスを用いた脳血流動態および脳酸素代謝の光イメージングシステムの構築2014

    • Author(s)
      西野明日香
    • Organizer
      脳循環代謝学会
    • Place of Presentation
      岡山(岡山コンベンションセンター)
    • Year and Date
      2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 覚醒マウスの脳機能におけるマルチモダルイメージング2014

    • Author(s)
      田桑 弘之
    • Organizer
      日本光学会
    • Place of Presentation
      東京(筑波大学)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Newly optical imaging systems of change in oxygen metabolism and hemodynamic using awake mice brain2014

    • Author(s)
      田桑 弘之
    • Organizer
      日本神経科学学会
    • Place of Presentation
      横浜(パシフィコ横浜)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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