| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The aim of this study is to investigate the mechanisms of taxane resistance using whole exon sequencing and expression analyses in human breast cancer tissues. First we chose six breast cancer patients whose tumors responded well to anthracycline treatment but suffered disease progression on taxane treatment. Next, we examined whole exon sequencing on these samples. As a result, we found some somatic mutations which were considered to be candidate genes involved in the resistance to taxanes. Then, we performed mRNA expression analyses of these candidate genes in 122 breast cancers treated with taxanes. We correlated mRNA expression levels of these genes with clinicopathological factors and prognosis.
We identified 9 mutations common to all 6 patients analyzed in this study, and a further 16 mutations shared by 5 of them. Kaplan-Meier analyses showed that high level mRNA expression of 3 of these 25 genes was significantly associated with poorer disease-free survival.
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