| Project/Area Number |
26861057
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Nagoya City University (2015)
Aichi Cancer Center Research Institute (2014) |
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Principal Investigator |
KONDO NAOTO 名古屋市立大学, 医学(系)研究科(研究院), 助教 (90529166)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANISHI HAYAO 愛知県がんセンター研究所, 腫瘍病理学部, 研究員 (20207830)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | breast cancer / CTC / filter / 乳癌 / 循環腫瘍細胞 |
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| Outline of Final Research Achievements |
We improved 3D filter type CTC isolation device of the rhinoceroses selectivity more and tried the improvement of the device to isolate it using a manipulator automatically, but it became clear for the adhesion of cells that quick aspiration, regurgitation by capirary were difficult.Therefore we canceled the single cells sampling of CTC with the manipulator and changed it to a method to copy CTC from a filter to object glass directly instead.The single cells sampling was not possible, but quickness, simple and easy CTC recovery were thereby enabled.Of few leukocytes contaminating it hardly influenced immunostaining and a genetic analysis.
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