| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Expression and functions of melanoma-associated antigen family D4 (MAGE-D4) was evaluated to identify novel molecular target of hepatocellular carcinoma (HCC). Five out of nine HCC cell lines, MAGE-D4 was remarkably overexpressed compared to control normal liver tissues. Knockdown of MAGE-D4 mediated by siRNA led to reduced migration and invasion abilities of HCC cells. No difference in the level of MAGE-D4 mRNA was found between three types of background liver (normal liver, chronic hepatitis and cirrhosis). MAGE-D4 protein was detected by immunohistochemical staining. Patients with high MAGE-D4 in HCC tissues had a significantly shorter survival. Expression of intra-tumoral MAGE-D4 had a correlation with VEGF, but not CD34 and HIF-1a. Some patients showed elevated serum MAGE-D4, however there were no correlations with AFP and PIVKA-II levels. MAGE-D4 may be a promising biomarker and therapeutic target of HCC.
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