| Project/Area Number |
26861091
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Saze Zenichiro 福島県立医科大学, 医学部, 助教 (10468126)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | MDSC / 制御性B細胞 / 抑制性B細胞 |
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| Outline of Final Research Achievements |
To verify whether low-density neutrophil cells have regulatory potential, we divided human PBMCs into low-density neutrophil cells and high-density cells. Then, we extracted CD15+ cells from each fraction, co-cultured with PBMCs under stimulation and measured INF-γ by ELISA. Suppression of INF-γ production was not observed in co-culture with high-density neutrophil cells. We were not able to examine suppression assay of co-culture with low-density neutrophil cells due to purity of CD15+ cells was low and the numbers of harvested low-density neutrophil cells from cancer patients were so small. Now we are trying to improve purity and quantity of low-density neutrophil cells. Using IHC, we analyzed correlation between CD15+ neutrophil in tumor and clinicopathological factors. No. of CD15+ cells of ly+ group was significantly higher than those of ly- group. We have plans to study about regulatory B cells from gastrointestinal cancer patients’ PBMC and tumor infiltrating lymphocytes.
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