| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
Immature dendritic cells contains the constitutive proteasome, however, mature dendritic cells differentiated from immature dendritic cells exposed with inflammatory mediator such as interferon-gamma contains immunoproteasome (ip) derived from immature dendritic cells. The inhibition of the immunoproteasome in mature dendritic cells using small interfering RNA transfection targeting the 3 inducible ip subunits might help to induce antigen-specific cytotoxic T lymphocytes. In this study, I performed basic research about anti-tumor immune response targeting mesothelin which is tumor associated antigen of pancreatic ductal carcinoma, and using dendritic cells transduced with mesothelin gene fused with ubiquitin gene which is a degradation signal of proteasome.
|
