Study on the malignancy and activity of stromal fibroblasts in colon cancer

• Project/Area Number: 26861097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: Ieda Junji 和歌山県立医科大学, 医学部, 学内助教 (50637907)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 大腸癌 / 線維芽細胞 / 間質

Outline of Final Research Achievements

Our previous study using a mammary fat pad mouse model showed that P4H9,produced by the b2 integrin epitope, detected a molecule on fibroblasts in response to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1)expressing cancer cells. P4H9-detected molecule (PDM) expression appeared to be associated with myofibroblast differentiation. In this study, we investigated whether PDM is expressed on fibroblasts and cancer cells in clinical tissue samples, and whether the presence of PDM-expressing colorectal cancer cells is correlated with clinicopathological features of patients. Multivariate analysis showed that PDM-expressing spindle-shaped fibroblasts were an independent risk factor for lymph node metastasis, hematogenous metastasis, and poor survival. A Kaplan-Meier survival curve indicated that PDM-expressing spindle-shaped fibroblasts were associated with shorter survival time (P<0.0001).

Research Products

• [Journal Article] P4H9-detected molecule expression on spindle-shaped fibroblasts indicates malignant phenotype of colorectal cancer (2015)
  • Author(s): Shozo Yokoyama, Junji Ieda, Naoyuki Yamamoto, Shunsuke Yamaguchi, Yasuyuki Mitani, Akihiro Takeuchi, Katsunari Takifuji, Tsukasa Hotta, Kenji Matsuda, Takashi Watanabe, John E Shively, Hiroki Yamaue
  • Journal Title: British Journal of Cancer Volume: 113 Issue: 10 Pages: 1454-1459
  • DOI: 10.1038/bjc.2015.363
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] CEACAM1 and hollow spheroid formation modulate the chemosensitivity of colorectal cancer to 5-fluorouracil. (2015)
  • Author(s): Yamamoto N, Yokoyama S, Ieda J, Mitani Y, Yamaguchi S, Takifuji K, Hotta T, Matsuda K, Watanabe T, Shively JE, Yamaue H.
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 75 Issue: 2 Pages: 421-430
  • DOI: 10.1007/s00280-014-2662-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma. (2014)
  • Author(s): Kiriyama S, Yokoyama S, Ueno M, Hayami S, Ieda J, Yamamoto N, Yamaguchi S, Mitani Y, Nakamura Y, Tani M, Mishra L, Shively JE, Yamaue H.
  • Journal Title: Annals of Surgical oncology Volume: Supple 4 Issue: S4 Pages: 505-514
  • DOI: 10.1245/s10434-013-3460-1
  • Peer Reviewed / Open Access
• [Presentation] 高齢大腸癌患者に対する総合的機能評価 (2015)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邉高士、三谷泰之、岩本博光、水本有紀、津村亜矢子、出口真彰、山上裕機
  • Organizer: 日本臨床外科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-26
• [Presentation] 下部直腸癌に対する腹腔鏡下内肛門括約筋切除術（ISR）に関する検討 (2015)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邊高士 三谷泰之、水本有紀、津村亜矢子、山上裕機
  • Organizer: 日本消化器外科学会
  • Place of Presentation: 浜松
  • Year and Date: 2015-07-15
• [Presentation] 75歳以上高齢者大腸癌術後補助化学療法に関する検討 (2015)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邊高士 三谷泰之、水本有紀、津村亜矢子、山上裕機
  • Organizer: 日本外科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-16
• [Presentation] 高齢者総合的機能評価(CGA)を用いた高齢者の腹腔鏡下大腸手術の安全性に関する検討 (2014)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邉高士、三谷泰之、水本有紀、津村亜矢子、山上裕機
  • Organizer: 臨床外科学会
  • Place of Presentation: 福島
  • Year and Date: 2014-11-22
• [Presentation] 腹腔鏡下手術で根治できたＳ状結腸膀胱瘻の３例 (2014)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邊高士、 三谷泰之、水本有紀、津村亜矢子、山上裕機
  • Organizer: 内視鏡外科学会
  • Place of Presentation: 盛岡
  • Year and Date: 2014-10-04
• [Presentation] 高齢者総合的機能評価(CGA)による高齢患者の腹腔鏡下大腸癌手術の安全性に関する検討 (2014)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邉高士、三谷泰之、水本有紀、山上裕機
  • Organizer: 消化器外科学会
  • Place of Presentation: 福島
  • Year and Date: 2014-07-16
• [Presentation] 75歳以上stageIII手術症例に対する術後補助化学療法に関する検討 (2014)
  • Author(s): 家田淳司、堀田 司、瀧藤克也、横山省三、松田健司、渡邊高士、三谷泰之、竹井 陽、水本有紀、津村亜矢子、山上裕機
  • Organizer: 大腸癌研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-07-07